Ali A M, Wright E G, Riches A C
Department of Biology & Preclinical Medicine, University of St Andrews, Scotland.
Cell Tissue Kinet. 1989 Jul;22(4):333-41. doi: 10.1111/j.1365-2184.1989.tb00218.x.
Changes in the kinetic state of pluripotent haemopoietic spleen colony forming cells (CFU-S) and of the CFU-S proliferation stimulator have been studied following whole-body X-irradiation. Rapid recruitment of CFU-S into cell cycle by 30 min after irradiation was observed following low doses (0.5 Gy) but a delay of 6 h occurred after higher doses (1.5 and 4.5 Gy). These changes in proliferative state correlated with the presence of the CFU-S proliferation stimulator. CFU-S irradiated in vitro in bone marrow plugs were also recruited into cycle illustrating directly the local nature of the feedback mechanism. CFU-S removed from 1.5 Gy irradiated recipients at a time when they were not in cycle were not responsive to the CFU-S proliferation stimulator. The CFU-S proliferation stimulator was produced by Ia positive cells in the irradiated bone marrow. The regulation changes occurring shortly after irradiation cannot simply be controlled by the size of the CFU-S compartment.
对全身X射线照射后多能造血脾集落形成细胞（CFU-S）的动力学状态变化以及CFU-S增殖刺激物进行了研究。低剂量（0.5 Gy）照射后30分钟观察到CFU-S迅速进入细胞周期，但高剂量（1.5和4.5 Gy）照射后出现6小时的延迟。增殖状态的这些变化与CFU-S增殖刺激物的存在相关。在骨髓塞中体外照射的CFU-S也进入细胞周期，直接说明了反馈机制的局部性质。在未处于细胞周期时从1.5 Gy照射的受体中取出的CFU-S对CFU-S增殖刺激物无反应。CFU-S增殖刺激物由照射骨髓中的Ia阳性细胞产生。照射后不久发生的调节变化不能简单地由CFU-S区室的大小控制。
