Nakamura M, Hamaguchi K, Ono J, Takaki R, Kitamura H, Nakamura H, Noguchi T, Ina K
Department of Anatomy, Medical College of Oita, Japan.
Diabetes Res Clin Pract. 1989 Sep 18;7(3):169-79. doi: 10.1016/0168-8227(89)90002-8.
We studied the microvasculature of the pancreatic islet with an electron microscope using 15 alloxan-diabetic and 16 control rats. These animals were morphologically examined at 2 weeks, 1, 2, and 4 months after alloxan injection (diabetic rats) or at comparable times (control rats). Control rats were non-diabetic, either sequentially treated with glucose and alloxan (injected controls) or not subjected to any medication (non-treated controls). Body weights, plasma glucose levels, and erythrocyte glycosylated hemoglobin (HbA1c) concentrations were also measured at intervals. All the diabetic rats exhibited retarded growth, hyperglycemia, and increases in HbA1c concentrations. The primary changes in diabetic islet capillaries can be grouped into four categories: (1) narrowing and closing of capillaries and occurrence of possible acellular capillaries, (2) formation of perivascular edema, (3) bulging and projection of endothelial cytoplasm, and (4) perivascular proliferation of collagen-like fibrils together with thickening of the basement membrane. These changes tended to worsen with diabetic duration. They are regarded as pathognomonic for diabetic microangiopathy. It is presumed that the present microvascular lesions of the islet play an important role in the pathogenesis and advancement of the diabetic state.
我们使用15只四氧嘧啶糖尿病大鼠和16只对照大鼠，通过电子显微镜研究了胰岛的微血管系统。在注射四氧嘧啶后2周、1个月、2个月和4个月（糖尿病大鼠）或在相应时间（对照大鼠）对这些动物进行形态学检查。对照大鼠为非糖尿病大鼠，要么依次用葡萄糖和四氧嘧啶处理（注射对照），要么未接受任何药物治疗（未处理对照）。还定期测量体重、血糖水平和红细胞糖化血红蛋白（HbA1c）浓度。所有糖尿病大鼠均表现出生长迟缓、高血糖和HbA1c浓度升高。糖尿病胰岛毛细血管的主要变化可分为四类：（1）毛细血管狭窄和闭合以及可能出现无细胞毛细血管；（2）血管周围水肿形成；（3）内皮细胞质膨出和突出；（4）血管周围胶原样纤维增生以及基底膜增厚。这些变化往往随着糖尿病病程的延长而恶化。它们被认为是糖尿病微血管病变的特征性表现。据推测，目前胰岛的微血管病变在糖尿病状态的发病机制和进展中起重要作用。
