Sommer Yuliya L, Ward Cynthia D, Pan Yi, Caldwell Kathleen L, Jones Robert L
Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, Inorganic and Radiation Analytical Toxicology Branch, 4770 Buford Highway NE, MS F-50, Atlanta, GA 30341, USA
Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, Inorganic and Radiation Analytical Toxicology Branch, 4770 Buford Highway NE, MS F-50, Atlanta, GA 30341, USA.
J Anal Toxicol. 2016 Apr;40(3):222-8. doi: 10.1093/jat/bkw007. Epub 2016 Feb 23.
In this study, we evaluated the effect of temperature on the long-term stability of three mercury species in bovine blood. We used inductively coupled plasma mass spectrometry (ICP-MS) analysis to determine the concentrations of inorganic (iHg), methyl (MeHg) and ethyl (EtHg) mercury species in two blood pools stored at temperatures of -70, -20, 4, 23°C (room temperature) and 37°C. Over the course of a year, we analyzed aliquots of pooled specimens at time intervals of 1, 2, 4 and 6 weeks and 2, 4, 6, 8, 10 and 12 months. We applied a fixed-effects linear model, step-down pairwise comparison and coefficient of variation statistical analysis to examine the temperature and time effects on changes in mercury species concentrations. We observed several instances of statistically significant differences in mercury species concentrations between different temperatures and time points; however, with considerations of experimental factors (such as instrumental drift and sample preparation procedures), not all differences were scientifically important. We concluded that iHg, MeHg and EtHg species in bovine whole blood were stable at -70, -20, 4 and 23°C for 1 year, but blood samples stored at 37°C were stable for no more than 2 weeks.
在本研究中，我们评估了温度对牛血中三种汞形态长期稳定性的影响。我们使用电感耦合等离子体质谱（ICP-MS）分析来测定储存在-70、-20、4、23°C（室温）和37°C的两个血库中无机汞（iHg）、甲基汞（MeHg）和乙基汞（EtHg）的浓度。在一年的时间里，我们在1、2、4和6周以及2、4、6、8、10和12个月的时间间隔分析混合样本的等分试样。我们应用固定效应线性模型、逐步两两比较和变异系数统计分析来研究温度和时间对汞形态浓度变化的影响。我们观察到不同温度和时间点之间汞形态浓度存在几个具有统计学意义的差异实例；然而，考虑到实验因素（如仪器漂移和样品制备程序），并非所有差异都具有科学重要性。我们得出结论，牛全血中的iHg、MeHg和EtHg形态在-70、-20、4和23°C下1年内是稳定的，但储存在37°C的血样稳定时间不超过2周。
