Pokorska Joanna, Kułaj Dominika, Dusza Magdalena, Żychlińska-Buczek Justyna, Makulska Joanna
a Department of Cattle Breeding , University of Agriculture in Krakow, Institute of Animal Science , Krakow , Poland.
Anim Biotechnol. 2016;27(2):113-7. doi: 10.1080/10495398.2015.1116446.
Isolation of genomic DNA is one of the basic steps in many different molecular analyses. There are a few reports on methods of DNA isolation from milk, but many of them are time consuming and expensive, and require relatively large volumes of raw milk. In this study a rapid, sensitive, and efficient method of DNA extraction from milk somatic cells of various mammals (cattle, sheep, goats, horses) is presented. It was found that milk is a good source of genomic DNA, and to obtain a sufficient amount and quality of DNA, suitable for molecular analysis such as PCR, 10 mL of raw milk is sufficient. Thanks to this method, stress in animals can be reduced during collection of researched material. Therefore, this method could be widely used in molecular analyses.
基因组DNA的分离是许多不同分子分析中的基本步骤之一。关于从牛奶中分离DNA的方法有一些报道，但其中许多方法既耗时又昂贵，而且需要相对大量的原料奶。在本研究中，提出了一种从各种哺乳动物（牛、羊、山羊、马）的牛奶体细胞中快速、灵敏且高效地提取DNA的方法。研究发现，牛奶是基因组DNA的良好来源，为获得足够数量和质量的、适用于PCR等分子分析的DNA，10毫升原料奶就足够了。由于这种方法，可以在采集研究材料时减轻动物的应激。因此，该方法可广泛应用于分子分析。
