Pokorska Joanna, Kułaj Dominika, Piestrzyńska-Kajtoch Agata, Radko Anna
Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Krakow, 30-059 Krakow, Poland.
Department of Animal Molecular Biology, The National Research Institute of Animal Production, 32-083 Balice, Poland.
Animals (Basel). 2019 Nov 18;9(11):992. doi: 10.3390/ani9110992.
In the recent years, antioxidant properties of food products have become an important aspect for consumers. Milk is a very good source of easily absorbable proteins and minerals, as well as a valuable source of antioxidants. Lipocalin-2 (LCN2), given that, inter alia, it is produced in large quantities by various types of cells in response to oxidative stress caused by physical or chemical factors, it can be considered a protein that determines the total antioxidant capacity of milk. The main objective of this study was to analyze polymorphisms within the lipocalin-2 gene and to determine their impact on antioxidant activity of milk from Holstein-Friesian cows. The genotyping was carried out by sequencing of PCR products. To determine the antioxidant activity of milk, the Trolox equivalent antioxidant capacity (TEAC) method was used. A total of four polymorphic sites were identified in the examined segment of the bovine lipocalin-2 gene. It was shown that cows of the CC genotype at the locus g.98793763G>C produced milk of significantly higher antioxidant capacity. The antioxidant capacity of milk also varied according to the age of cows, their daily milk yield, and SCC in milk.
近年来，食品的抗氧化特性已成为消费者关注的一个重要方面。牛奶是易吸收蛋白质和矿物质的优质来源，也是抗氧化剂的宝贵来源。鉴于脂联素-2（LCN2）尤其能由各类细胞在受到物理或化学因素引起的氧化应激时大量产生，它可被视为一种决定牛奶总抗氧化能力的蛋白质。本研究的主要目的是分析脂联素-2基因内的多态性，并确定它们对荷斯坦-弗里生奶牛所产牛奶抗氧化活性的影响。通过对PCR产物进行测序来进行基因分型。为测定牛奶的抗氧化活性，采用了特洛克斯等效抗氧化能力（TEAC）方法。在牛脂联素-2基因的检测片段中共鉴定出四个多态性位点。结果表明，在g.98793763G>C位点具有CC基因型的奶牛所产牛奶的抗氧化能力显著更高。牛奶的抗氧化能力还会因奶牛的年龄、日产奶量以及牛奶中的体细胞数而有所不同。
