Environmental Engineering and Management Research Group, Ton Duc Thang University, Ho Chi Minh City, Viet Nam; Faculty of Environment and Labor Safety, Ton Duc Thang University, Ho Chi Minh City, Viet Nam.
Faculty of Environment and Natural Resources, University of Technology, Vietnam National University - Ho Chi Minh, District 10, Ho Chi Minh City, Viet Nam.
Bioresour Technol. 2016 May;208:1-6. doi: 10.1016/j.biortech.2016.02.043. Epub 2016 Feb 15.
One of the buffers namely Tris (Tris-(hydroxymethyl)-amino methane) was used to increase the growth of microalgae by stabilizing the pH value in microalgae cultures. The objective of this research is to determine the growth rate and biomass productivity of Chlorella sp. with and without Tris addition. Both conditions function at various N:P ratios cultured in photobioreactors (carbon dioxide of 5%(v/v), light intensity of 3.3 Klux). Daily variations in nutrient removal (nitrogen and phosphorus), cell concentration, DO, temperature and pH were measured for data analysis. The results show that the largest yield of biomass was achieved at the N:P ratio of 15:1 with and without Tris. After cultivation lasting 92 h, the algae concentration at this ratio was 1250 mg L(-1) and 3568 mg L(-1) with and without Tris, respectively. This indicates that adding Tris to the photobioreactor greatly reduces algae biomass due to bacterial competition.
其中一种缓冲剂，即 Tris（三羟甲基氨基甲烷），通过稳定微藻培养物中的 pH 值来促进微藻的生长。本研究的目的是确定添加和不添加 Tris 时小球藻的生长率和生物量生产力。在光生物反应器中（二氧化碳 5％（v/v），光照强度 3.3 Klux），在不同的 N：P 比下培养两种条件。为了进行数据分析，测量了营养物去除（氮和磷）、细胞浓度、DO、温度和 pH 值的日变化。结果表明，在 N：P 比为 15：1 时，添加和不添加 Tris 均可获得最大的生物量产量。培养 92 小时后，该比例下的藻类浓度分别为 1250 mg L(-1)和 3568 mg L(-1)，添加和不添加 Tris。这表明，向光生物反应器中添加 Tris 会由于细菌竞争而大大降低藻类生物量。
