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农业果皮废弃物混合培养发酵生产乳酸过程中微生物群落变化分析。

Analysis of microbial community variation during the mixed culture fermentation of agricultural peel wastes to produce lactic acid.

机构信息

Renewable Materials Program, Department of Forest, Rangeland and Fire Sciences, University of Idaho, 875 Perimeter Drive MS1132, Moscow, ID 83844, United States.

Department of Biological Sciences and the Institute for Bioinformatics and Evolutionary Studies, University of Idaho, Moscow, ID 83844, United States.

出版信息

Bioresour Technol. 2016 May;208:7-12. doi: 10.1016/j.biortech.2016.02.054. Epub 2016 Feb 19.

DOI:10.1016/j.biortech.2016.02.054
PMID:26913642
Abstract

Mixed cultures fermentation can be used to convert organic wastes into various chemicals and fuels. This study examined the fermentation performance of four batch reactors fed with different agricultural (orange, banana, and potato (mechanical and steam)) peel wastes using mixed cultures, and monitored the interval variation of reactor microbial communities with 16S rRNA genes using Illumina sequencing. All four reactors produced similar chemical profile with lactic acid (LA) as dominant compound. Acetic acid and ethanol were also observed with small fractions. The Illumina sequencing results revealed the diversity of microbial community decreased during fermentation and a community of largely lactic acid producing bacteria dominated by species of Lactobacillus developed.

摘要

混合培养发酵可用于将有机废物转化为各种化学物质和燃料。本研究使用混合培养物考察了四个批次反应器在不同农业(橙、香蕉和土豆(机械和蒸汽)皮废物)进料下的发酵性能,并使用 Illumina 测序监测了反应器微生物群落的间隔变化 16S rRNA 基因。四个反应器均产生类似的化学特征,以乳酸(LA)为主。也观察到少量的乙酸和乙醇。Illumina 测序结果表明,微生物群落的多样性在发酵过程中降低,主要由乳杆菌属物种组成的产大量乳酸的细菌群落占主导地位。

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