Nistal E, Caminero A, Herrán A R, Pérez-Andres J, Vivas S, Ruiz de Morales J M, Sáenz de Miera L E, Casqueiro J
Área de Microbiología, Facultad de Biología y Ciencias Ambientales, Universidad de León, León, Spain.
Instituto de Biología Molecular, Genómica y Proteómica (INBIOMIC), Universidad de León, León, Spain.
J Appl Microbiol. 2016 Jun;120(6):1691-700. doi: 10.1111/jam.13111. Epub 2016 Apr 4.
Several studies have suggested that abnormalities in the small-intestinal microbiota might be involved in the development or the pathogenesis of celiac disease (CD). The objective of this study was to characterize and compare the composition of the duodenal microbiota between CD patients and non-CD controls.
Bacterial communities were identified by pyrosequencing of 16S rRNA extracted from duodenal biopsies. The sequences analysis showed that the majority of the reads were classified within two phyla: Firmicutes and Proteobacteria. Bacterial richness and diversity were higher in non-CD controls than in untreated CD patients, but the differences were not statistically significant. The principal coordinates analysis revealed that bacterial communities of non-CD controls and untreated CD patients were dispersed without forming a clear group according to diagnosis of CD.
There are no statistically significant differences in the upper small intestinal composition of bacterial communities between untreated CD patients and non-CD controls.
This pyrosequencing analysis reveals a global picture of the duodenal microbiota that could be useful in future trials investigating the role of the microbiota in CD.
多项研究表明，小肠微生物群异常可能参与乳糜泻（CD）的发生或发病机制。本研究的目的是对CD患者和非CD对照者的十二指肠微生物群组成进行表征和比较。
通过对十二指肠活检组织中提取的16S rRNA进行焦磷酸测序来鉴定细菌群落。序列分析表明，大多数读数归类于两个门：厚壁菌门和变形菌门。非CD对照者的细菌丰富度和多样性高于未经治疗的CD患者，但差异无统计学意义。主坐标分析显示，非CD对照者和未经治疗的CD患者的细菌群落分散分布，未根据CD诊断形成明显的分组。
未经治疗的CD患者和非CD对照者的上小肠细菌群落组成无统计学显著差异。
这种焦磷酸测序分析揭示了十二指肠微生物群的整体情况，这可能有助于未来研究微生物群在CD中作用的试验。
