Yu Yi, Yang Qi-chang, Liu Wen-ke
Ying Yong Sheng Tai Xue Bao. 2015 Nov;26(11):3361-6.
Purple lettuce was grown hydroponically under six different nitrogen nutrition conditions, with NO(3-)-N:NH(4+)-N at 1:0, 4:1 and 1:1 combined with nitrogen application levels of 10 and 15 mmol · L(-1), for 25 days in solar greenhouse, then treated with short-term continuous lighting (SCL) before harvest to study the changes in contents of nutrients and analyze the effects of nitrogen nutrition conditions on the changes. Results showed that the shoot dry mass of all six nitrogen nutrition conditions were significantly improved under SCL treatment, by 35.1% at least, and the root dry mass increased greatly except for NO(3-)-N:NH(4+)-N 1:1 combined with nitrogen application level 15 mmol · L(-1) treatment and NO(3-)-N:NH(4+)-N 1:0 combined with nitrogen application level 10 mmol · L(-1) treatment. The relative contents of total phenols and flavonoid of different nitrogen nutrition conditions turned significantly different after treatment with SCL. The relative contents of total phenols and flavonoid were enhanced with the improvement of ammonium nitrogen ratio, while the relative content of anthocyanin increased and then decreased with the improvement of ammonium nitrogen ratio. The lighting treatment reduced the nitrate content of leaf blade of all six nitrogen nutrition conditions remarkably by 23.2% at least. The contents of ascorbic acid, soluble sugar and soluble protein rose significantly under SCL treatments. The study showed that the reduction of nitrate content speeded up with the enhancement of nitrogen application level and ammonium nitrogen ratio, and the advancement of ascorbic acid content slowed down with the increasing nitrogen application level. The soluble sugar improvement speed increased with the increasing ammonium nitrogen ratio, and SCL lifted the dry mass of the lettuce greatly. The results showed that SCL with LED lamps improved significantly the dry matter of lettuce under different nitrogen nutrition conditions, reduced the nitrate content and increased the ascorbic acid, soluble sugar and soluble protein contents greatly. In addition, nitrogen nutrition conditions affected the effectiveness of short-term continuous lighting on quality improvement rate of hydroponic lettuce remarkably.
紫色生菜在六种不同氮素营养条件下进行水培,其中硝态氮(NO₃⁻-N)与铵态氮(NH₄⁺-N)比例分别为1:0、4:1和1:1,并结合10和15 mmol·L⁻¹的施氮水平,在日光温室内培养25天,收获前进行短期连续光照(SCL)处理,以研究养分含量变化并分析氮素营养条件对这些变化的影响。结果表明,在SCL处理下,所有六种氮素营养条件下的地上部干质量均显著提高,至少提高了35.1%,除了硝态氮(NO₃⁻-N)与铵态氮(NH₄⁺-N)比例为1:1且施氮水平为15 mmol·L⁻¹的处理以及硝态氮(NO₃⁻-N)与铵态氮(NH₄⁺-N)比例为1:0且施氮水平为10 mmol·L⁻¹的处理外,根部干质量也大幅增加。不同氮素营养条件下总酚和类黄酮的相对含量在SCL处理后有显著差异。总酚和类黄酮的相对含量随着铵态氮比例的提高而增加,而花青素的相对含量随着铵态氮比例的提高先增加后降低。光照处理使所有六种氮素营养条件下叶片的硝酸盐含量显著降低,至少降低了23.2%。在SCL处理下,抗坏血酸、可溶性糖和可溶性蛋白质的含量显著上升。研究表明,硝酸盐含量的降低随着施氮水平和铵态氮比例的提高而加快,抗坏血酸含量的增加随着施氮水平的提高而减缓。可溶性糖含量的提高速度随着铵态氮比例的增加而加快,SCL显著提高了生菜的干质量。结果表明,采用LED灯的SCL显著提高了不同氮素营养条件下生菜的干物质含量,降低了硝酸盐含量,并大幅提高了抗坏血酸、可溶性糖和可溶性蛋白质的含量。此外,氮素营养条件显著影响短期连续光照对水培生菜品质改善率的效果。