用于定量测定人血浆和仓鼠血浆中米替福新的液相色谱-电喷雾串联质谱法
LC-coupled ESI MS for quantification of miltefosine in human and hamster plasma.
作者信息
Jaiswal Swati, Sharma Abhisheak, Shukla Mahendra, Lal Jawahar
机构信息
Pharmacokinetics & Metabolism Division, CSIR-Central Drug Research Institute, Lucknow 226031, India.
Academy of Scientific & Innovative Research, New Delhi, India.
出版信息
Bioanalysis. 2016 Mar;8(6):533-45. doi: 10.4155/bio.16.7. Epub 2016 Feb 26.
BACKGROUND
Leishmaniasis, a fatal parasitic disease, is the second largest parasitic killer in the world and miltefosine is the first and only oral drug available for its treatment.
RESULTS
A rapid, sensitive and simple LC-MS/MS method for miltefosine quantification in hamster and human plasma was developed and validated over the range of 2.5-400 ng/ml. The mass spectrometric detection of the drug was carried out in multiple reaction monitoring mode (m/z 408.1→125.1) using an electrospray positive ionization. The protein precipitation method was employed for sample (50 µl) cleanup.
CONCLUSION
The proposed method provided accurate and precise high-throughput quantification of miltefosine in plasma and was successfully applied to its oral PK study in Golden Syrian hamsters.
背景
利什曼病是一种致命的寄生虫病，是世界上第二大寄生虫杀手，而米替福新是第一种也是唯一一种可用于治疗该病的口服药物。
结果
开发并验证了一种快速、灵敏且简单的液相色谱-串联质谱法，用于在仓鼠和人血浆中定量米替福新，定量范围为2.5 - 400 ng/ml。采用电喷雾正离子化，在多反应监测模式（m/z 408.1→125.1）下对该药物进行质谱检测。采用蛋白质沉淀法对50 μl样品进行净化处理。
结论
所提出的方法可对血浆中的米替福新进行准确、精密的高通量定量，并成功应用于其在金黄叙利亚仓鼠中的口服药代动力学研究。
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