Xian Wenjing, Wu Yan, Xiong Wei, Li Longyan, Li Tong, Pan Shangwen, Song Limin, Hu Lisha, Pei Lei, Yao Shanglong, Shang You
Department of Anesthesiology, Institute of Anesthesiology and Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Biochem Biophys Res Commun. 2016 Mar 25;472(1):175-81. doi: 10.1016/j.bbrc.2016.02.090. Epub 2016 Feb 23.
Inflammation plays a crucial role in acute ischemic stroke pathogenesis. Macrophage-derived Maresin 1 (MaR1) is a newly uncovered mediator with potent anti-inflammatory abilities. Here, we investigated the effect of MaR1 on acute inflammation and neuroprotection in a mouse brain ischemia reperfusion (I/R) model. Male C57 mice were subjected to 1-h middle cerebral artery occlusion (MCAO) and reperfusion. By the methods of 2,3,5-triphenyltetrazolium chloride, haematoxylin and eosin or Fluoro-Jade B staining, neurological deficits scoring, ELISA detection, immunofluorescence assay and western blot analysis, we found that intracerebroventricular injection of MaR1 significantly reduced the infarct volume and neurological defects, essentially protected the brain tissue and neurons from injury, alleviated pro-inflammatory reactions and NF-κB p65 activation and nuclear translocation. Taken together, our results suggest that MaR1 significantly protects against I/R injury probably by inhibiting pro-inflammatory reactions.
炎症在急性缺血性脑卒中发病机制中起关键作用。巨噬细胞衍生的maresin 1（MaR1）是一种新发现的具有强大抗炎能力的介质。在此，我们在小鼠脑缺血再灌注（I/R）模型中研究了MaR1对急性炎症和神经保护的作用。雄性C57小鼠接受1小时大脑中动脉闭塞（MCAO）并再灌注。通过2,3,5-三苯基四氮唑氯化物、苏木精和伊红或Fluoro-Jade B染色、神经功能缺损评分、ELISA检测、免疫荧光分析和蛋白质印迹分析等方法，我们发现脑室内注射MaR1可显著减少梗死体积和神经功能缺损，从根本上保护脑组织和神经元免受损伤，减轻促炎反应以及NF-κB p65的激活和核转位。综上所述，我们的结果表明MaR1可能通过抑制促炎反应显著保护免受I/R损伤。
