Dunger A, Lucke S, Klöting I, Besch W, Hahn H J
Central Institute of Diabetes Gerhardt Katsch, Department of Experimental Diabetes Research, Karlsburg, GDR.
Int J Pancreatol. 1989 Dec;5(4):379-86. doi: 10.1007/BF02924301.
Neonatal pancreatic rat islets were used to investigate the effect of MHC haplotype RT1u on islet replication measured by incorporation of 3H-thymidine and autoradiography. The special interest in RT1u resulted from the fact that BB rats, which develop an insulin-dependent diabetes, belong to the RT1u haplotype. Thus, the RT1u, when influencing the replication, may be involved in the development of the B-cell deficiency observed in diabetic rats. The incorporation of labeled thymidine into islets obtained from rat strains carrying the RT1u or RTIa haplotype revealed no differences. No matter whether the islets were isolated from Lewis, Wistar, or BB rats, no alteration of islet replication by RT1u islets was found. However, islets obtained from Wistar rats had a higher replicatory activity than islets from Lewis rats. This finding was confirmed by a decrease in DNA synthesis of BB rat islets, in which the Wistar background was substituted by the Lewis background. We conclude from our results that MHC has no influence on islet replication, whereas genetic background seems to be involved in the regulation of islet DNA synthesis.
采用新生大鼠的胰腺胰岛，通过掺入³H-胸腺嘧啶核苷和放射自显影法来研究主要组织相容性复合体（MHC）单倍型RT1u对胰岛复制的影响。对RT1u特别感兴趣是因为患胰岛素依赖型糖尿病的BB大鼠属于RT1u单倍型。因此，RT1u若影响复制，可能与糖尿病大鼠中观察到的B细胞缺陷的发生有关。将标记的胸腺嘧啶核苷掺入携带RT1u或RTIa单倍型的大鼠品系的胰岛中，未发现差异。无论胰岛是从刘易斯大鼠、Wistar大鼠还是BB大鼠中分离得到的，均未发现RT1u胰岛对胰岛复制有改变。然而，从Wistar大鼠获得的胰岛比从刘易斯大鼠获得的胰岛具有更高的复制活性。用刘易斯背景取代Wistar背景的BB大鼠胰岛的DNA合成减少，证实了这一发现。我们从结果得出结论，MHC对胰岛复制没有影响，而遗传背景似乎参与了胰岛DNA合成的调节。
