Kumar Ashwani, Ghosh Biplab, Poswal H K, Pandey K K, Hosur M V, Dwivedi Abhilash, Makde Ravindra D, Sharma Surinder M
High Pressure and Synchrotron Radiation Physics Division, Bhabha Atomic Research Center, Trombay, Mumbai, India.
Advanced Centre for Treatment, Research and Education in Cancer, Navi Mumbai, India.
J Synchrotron Radiat. 2016 Mar;23(2):629-34. doi: 10.1107/S160057751600076X. Epub 2016 Feb 13.
The protein crystallography beamline (PX-BL21), installed at the 1.5 T bending-magnet port at the Indian synchrotron (Indus-2), is now available to users. The beamline can be used for X-ray diffraction measurements on a single crystal of macromolecules such as proteins, nucleic acids and their complexes. PX-BL21 has a working energy range of 5-20 keV for accessing the absorption edges of heavy elements commonly used for phasing. A double-crystal monochromator [Si(111) and Si(220)] and a pair of rhodium-coated X-ray mirrors are used for beam monochromatization and manipulation, respectively. This beamline is equipped with a single-axis goniometer, Rayonix MX225 CCD detector, fluorescence detector, cryogenic sample cooler and automated sample changer. Additional user facilities include a workstation for on-site data processing and a biochemistry laboratory for sample preparation. In this article the beamline, other facilities and some recent scientific results are briefly described.
安装在印度同步加速器（Indus-2）1.5 T弯铁端口的蛋白质晶体学光束线（PX-BL21）现已向用户开放。该光束线可用于对蛋白质、核酸及其复合物等大分子单晶进行X射线衍射测量。PX-BL21的工作能量范围为5-20 keV，用于获取常用于相位分析的重元素的吸收边。分别使用双晶单色器[Si(111)和Si(220)]和一对镀铑X射线镜进行光束单色化和光束操控。该光束线配备了单轴测角仪、Rayonix MX225 CCD探测器、荧光探测器、低温样品冷却器和自动样品更换器。其他用户设施包括用于现场数据处理的工作站和用于样品制备的生物化学实验室。本文简要介绍了该光束线、其他设施以及一些近期的科学成果。
