Mitsuhashi N, Kazumoto T, Nakayama Y, Sugiyama S, Yamakawa M, Yonome I, Nakamura Y, Nagai T, Niibe H
Dept. of Radiology, Gunma Univ. School of Med.
Gan No Rinsho. 1989 Dec;35(15):1753-8.
To determine if IMACIS-1 might have the capability of specifically pinpointing an adenocarcinoma in the human lung, xenografts of adenocarcinomatous nude mice that were injected with IMACIS-1 have been evaluated by scintigraphy, and the IMACIS-1 biodistribution in the tissue measured after sacrifice. Results have revealed that scintigraphic images obtained twenty-four hours after the IMACIS-1 injection showed some activity in the area of the tumor. At seventy-two hours after the IMACIS-1 injection, tumoral radioactivity was only seen in mice with a large tumor. At ninety-two hours after injection, the animals were sacrificed and a biodistribution study was performed. The IMACIS-1 uptake was expressed in counts per minute per gram of tissue. The tissue-to-blood uptake ratio in mice with a large tumor was 8.4 in the viable part of the tumor, 27.2 in the necrotic part of the tumor, 3.0 in the liver, and 1.5 in the spleen, respectively. In contrast, the ratio in mice with a small tumor was 3.4 in the viable part and 18.3 in the necrotic part. Immunoperoxidase staining with either the anti CEA or the anti CA 19-9 antibody was strongly visible in the necrotic part of the tumor.
为了确定IMACIS - 1是否具有特异性定位人肺腺癌的能力，已通过闪烁扫描对注射了IMACIS - 1的腺癌裸鼠异种移植物进行了评估，并在处死后测量了IMACIS - 1在组织中的生物分布。结果显示，注射IMACIS - 1后24小时获得的闪烁扫描图像显示肿瘤区域有一些活性。注射IMACIS - 1后72小时，仅在肿瘤较大的小鼠中观察到肿瘤放射性。注射后92小时，处死动物并进行生物分布研究。IMACIS - 1摄取以每克组织每分钟计数表示。肿瘤较大的小鼠中，肿瘤存活部分的组织与血液摄取比为8.4，肿瘤坏死部分为27.2，肝脏为3.0，脾脏为1.5。相比之下，肿瘤较小的小鼠中，存活部分的比值为3.4，坏死部分为18.3。用抗CEA或抗CA 19 - 9抗体进行的免疫过氧化物酶染色在肿瘤坏死部分强烈可见。
