SPOCK1 过表达与膀胱癌不良预后相关。
SPOCK1 Overexpression Confers a Poor Prognosis in Urothelial Carcinoma.
机构信息
1. Department of Pathology, Chi-Mei Medical Center, Tainan, Taiwan;
2. Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan;; 3. Department of Urology, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan;; 4. Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan;; 5. Department of Urology, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung, Taiwan;; 6. Center for Stem Cell Research, Kaohsiung Medical University, Kaohsiung, Taiwan;
出版信息
J Cancer. 2016 Jan 29;7(4):467-76. doi: 10.7150/jca.13625. eCollection 2016.
PURPOSE
The majority deaths of cancer patients are related to metastasis, thus genes associated with cell motility interest us. SPOCK1 was elected by data mining and serial evaluation. In addition, SPOCK1 has been reported to be highly expressed in different human cancers and been related to adverse outcomes. Therefore, we validate its prognostic significance in urothelial carcinoma (UC).
MATERIALS AND METHODS
Real-time RT-PCR assay was used to detect SPOCK1 transcript level in 27 urinary tract urothelial carcinoma (UTUC) and 27 urinary bladder urothelial carcinoma (UBUC) samples. Immunohistochemistry evaluated by H-score determined SPOCK1 expressions in 340 UTUCs and 295 UBUCs. The transcript and protein expression were correlated with clinicopathological features. Further evaluations of the prognostic significance of SPOCK1 for disease-specific survival (DSS) and metastasis-free survival (MeFS) were analyzed.
RESULTS
The expressions of SPOCK1 in UC were higher than those in normal urothelium by immunohistochemistry. The statistical analysis of clinicopathologic characteristics and immunohistochemistry showed that the higher expression of SPOCK1 was correlated to pT status (P<0.001), lymph node metastasis (UTUC, P=0.006; UBUC, P=0.033), higher histological grade (UTUC, P<0.001; UBUC, P<0.001), vascular invasion (UTUC, P<0.001; UBUC, P<0.001), perineurial invasion (UTUC, P<0.001; UBUC, P=0.001) and frequent mitosis (UTUC, P<0.001; UBUC, P=0.001). The prognosis of SPOCK1 of UC showed high SPOCK1 expression had significantly worse DSS and MeFS.
CONCLUSIONS
The investigation demonstrated that the higher expression of SPOCK1 correlates with a poor prognosis in UC.
目的
大多数癌症患者的死亡与转移有关，因此与细胞迁移相关的基因引起了我们的兴趣。SPOCK1 通过数据挖掘和连续评估被选中。此外，已有报道称 SPOCK1 在不同的人类癌症中高度表达，并与不良预后相关。因此，我们验证了其在尿路上皮癌（UC）中的预后意义。
材料和方法
使用实时 RT-PCR 检测 27 例尿路上皮癌（UTUC）和 27 例膀胱癌（UBUC）样本中的 SPOCK1 转录水平。免疫组织化学通过 H 评分评估 SPOCK1 在 340 例 UTUC 和 295 例 UBUC 中的表达。对转录和蛋白表达与临床病理特征进行相关性分析。进一步分析 SPOCK1 对疾病特异性生存（DSS）和无转移生存（MeFS）的预后意义。
结果
免疫组化显示 UC 中的 SPOCK1 表达高于正常尿路上皮。对临床病理特征和免疫组化的统计分析表明，SPOCK1 的高表达与 pT 分期（P<0.001）、淋巴结转移（UTUC，P=0.006；UBUC，P=0.033）、较高的组织学分级（UTUC，P<0.001；UBUC，P<0.001）、血管侵犯（UTUC，P<0.001；UBUC，P<0.001）、神经周围侵犯（UTUC，P<0.001；UBUC，P=0.001）和频繁有丝分裂（UTUC，P<0.001；UBUC，P=0.001）相关。UC 的 SPOCK1 预后显示，高 SPOCK1 表达与 DSS 和 MeFS 显著较差相关。
结论
本研究表明，SPOCK1 的高表达与 UC 的不良预后相关。
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