Ncube K T, Mdladla K, Dzomba E F, Muchadeyi F C
Biotechnology Platform, Agricultural Research Council, Private Bag X5, Onderstepoort, 0110, South Africa.
Discipline of Genetics, University of KwaZulu-Natal, School of Life Sciences, P.O. Box X01, Scottsville, Pietermaritzburg, 3209, South Africa.
Anim Genet. 2016 Jun;47(3):382-5. doi: 10.1111/age.12424. Epub 2016 Feb 25.
This study assessed the genetic diversity in the growth hormone 1 gene (GH1) within and between South African goat breeds. Polymerase chain reaction-targeted gene amplification together with Illumina MiSeq next-generation sequencing (NGS) was used to generate the full length (2.54 kb) of the growth hormone 1 gene and screen for SNPs in the South African Boer (SAB) (n = 17), Tankwa (n = 15) and South African village (n = 35) goat populations. A range of 27-58 SNPs per population were observed. Mutations resulting in amino acid changes were observed at exons 2 and 5. Higher within-breed diversity of 97.37% was observed within the population category consisting of SA village ecotypes and the Tankwa goats. Highest pairwise FST values ranging from 0.148 to 0.356 were observed between the SAB and both the South African village and Tankwa feral goat populations. Phylogenetic analysis indicated nine genetic clusters, which reflected close relationships between the South African populations and the other international breeds with the exception of the Italian Sarda breeds. Results imply greater potential for within-population selection programs, particularly with SA village goats.
本研究评估了南非山羊品种内部以及不同品种之间生长激素1基因（GH1）的遗传多样性。采用聚合酶链反应靶向基因扩增技术结合Illumina MiSeq下一代测序（NGS），以生成生长激素1基因的全长（2.54 kb），并对南非布尔山羊（SAB，n = 17）、坦克瓦山羊（n = 15）和南非乡村山羊（n = 35）群体进行单核苷酸多态性（SNP）筛选。每个群体观察到的SNP数量在27至58个之间。在第2外显子和第5外显子处观察到导致氨基酸变化的突变。在由南非乡村生态型山羊和坦克瓦山羊组成的群体类别中，观察到较高的品种内多样性，为97.37%。在南非布尔山羊与南非乡村山羊和坦克瓦野生山羊群体之间观察到最高的成对FST值，范围为0.148至0.356。系统发育分析表明存在9个遗传簇，这反映了南非群体与其他国际品种之间的密切关系，但意大利撒丁岛品种除外。结果表明群体内选择计划具有更大潜力，特别是对于南非乡村山羊。
