Fazio Esterina, Medica Pietro, Cravana Cristina, Bruschetta Giuseppe, Ferlazzo Adriana
Department of Veterinary Sciences, Unit of Veterinary Physiology, University of Messina, Messina, Italy.
Department of Veterinary Sciences, Unit of Veterinary Physiology, University of Messina, Messina, Italy.
Theriogenology. 2016 Jun;85(9):1582-1589. doi: 10.1016/j.theriogenology.2016.01.017. Epub 2016 Jan 29.
The purpose of this study was to determine which physiological seasonal thyroid and lipid panel (triglyceride, [TG], total cholesterol, [tCho], high-density lipoprotein cholesterol, [HDL Cho], LDL cholesterol, phospholipids, [PDs]) changes occur in 12 Thoroughbred pregnant and six nonpregnant mares over a period of 12 months, from April to March, by into account the lactation stage. Serum total triiodothyronine (T3) and thyroxine (T4) levels were measured using immunoenzymatic assay kits; the serum lipid panel was analyzed using the enzymatic colorimetric method. One-way ANOVA reported a significant effect of time over 12 months for T3 (F = 5.46; P < 0.001) and T4 (F = 3.98; P < 0.001), in both pregnant and nonpregnant mares, and for triglyceride (F = 2.57; P < 0.01), tCho (F = 2.32; P < 0.01), HDL Cho (F = 5.60; P < 0.0001), and PDs (F = 7.01; P < 0.0001) in pregnant mares. Two-way ANOVA repoted a significant lactating effect compared with nonlactating stage for T3 (F = 8.33; P = 0.006), T4 (F = 6.43; P = 0.003), tCho (F = 10.38; P = 0.0078), HDL Cho (F = 7.64; P = 0.020), and PDs (F = 5.07; P = 0.048). Thus, it appears that under similar environmental condition, nutrition regime, and management system, lactation stage plays a significant role in the seasonal thyroid and lipid profiles in Thoroughbred pregnant mares, with higher T3, T4, and PDs values, and lower tCho and HDL Cho in lactating than nonlactating mares. The physiological values obtained in this study may be used as additional resources to evaluate thyroid and lipid profiles in Thoroughbred pregnant and nonpregnant mares, generating a wished numbers of observation, especially when the additional breed and physiological conditions are considered.
本研究的目的是确定在12个月（从4月至次年3月）的时间里，12匹纯种怀孕母马和6匹未怀孕母马的生理季节性甲状腺和血脂指标（甘油三酯、[TG]、总胆固醇、[tCho]、高密度脂蛋白胆固醇、[HDL Cho]、低密度脂蛋白胆固醇、磷脂、[PDs]）会发生哪些变化，并考虑泌乳阶段。使用免疫酶测定试剂盒测量血清总三碘甲状腺原氨酸（T3）和甲状腺素（T4）水平；采用酶比色法分析血清血脂指标。单因素方差分析表明，在怀孕和未怀孕母马中，12个月内时间对T3（F = 5.46；P < 0.001）和T4（F = 3.98；P < 0.001）有显著影响，对怀孕母马的甘油三酯（F = 2.57；P < 0.01）、tCho（F = 2.32；P < 0.01）、HDL Cho（F = 5.60；P < 0.0001）和PDs（F = 7.01；P < 0.0001）也有显著影响。双因素方差分析表明，与非泌乳阶段相比，泌乳阶段对T3（F = 8.33；P = 0.006）、T4（F = 6.43；P = 0.003）、tCho（F = 10.38；P = 0.0078）、HDL Cho（F = 7.64；P = 0.020）和PDs（F = 5.07；P = 0.048）有显著影响。因此，在相似的环境条件、营养方案和管理系统下，泌乳阶段对纯种怀孕母马的季节性甲状腺和血脂谱有显著影响，泌乳母马比非泌乳母马的T3、T4和PDs值更高，tCho和HDL Cho值更低。本研究获得的生理值可作为评估纯种怀孕和未怀孕母马甲状腺和血脂谱的额外资源，尤其是在考虑额外的品种和生理条件时，可产生理想数量的观察结果。
