[使用免疫酶和镧系元素免疫荧光分析在临床样本中检测流感病毒]

[Indication of the influenza virus in clinical samples using immunoenzyme and lanthanide immunofluorescence analyses].

作者信息

Khristova M L, Busse T L, Zagidullin N V, Leonov S V, Rybalko S L, Frolov A F, Kharitonenkov I G

出版信息

Vopr Virusol. 1989 Sep-Oct;34(5):538-42.

Abstract

A comparative study of the potentials of enzyme-immunoassay (EIA) and lanthanide immunofluorescent assay (LIFA) for influenza virus detection in nasopharyngeal aspirates and nasopharyngeal washings was carried out. Monospecific and polyclonal antisera to hemagglutinin polyclonal antisera to matrix protein as well as polyclonal and monoclonal antisera to nucleoprotein were used. The nasopharyngeal aspirates were shown to contain virus-specific antigens in the amounts sufficient for influenza virus detection with polyclonal and monoclonal antibodies. There was a good correlation between chromophore results by EIA and fluorescence level in LIFA. The sensitivity of the test systems used (EIA and LIFA) was shown to be insufficient for the detection of virus-specific material in the nasopharyngeal washings. Besides, there was no correlation between the results obtained by different test systems. It was concluded that nasopharyngeal aspirates should be collected for rapid influenza diagnosis by EIA and LIFA.

摘要

开展了一项关于酶免疫测定法（EIA）和镧系元素免疫荧光测定法（LIFA）检测鼻咽抽吸物和鼻咽冲洗液中流感病毒潜力的比较研究。使用了针对血凝素的单特异性和多克隆抗血清、针对基质蛋白的多克隆抗血清以及针对核蛋白的多克隆和单克隆抗血清。结果显示，鼻咽抽吸物中含有足以用多克隆和单克隆抗体检测流感病毒的病毒特异性抗原。EIA的发色团结果与LIFA的荧光水平之间存在良好的相关性。所使用的检测系统（EIA和LIFA）的灵敏度不足以检测鼻咽冲洗液中的病毒特异性物质。此外，不同检测系统获得的结果之间没有相关性。得出的结论是，应收集鼻咽抽吸物用于通过EIA和LIFA进行快速流感诊断。