Busse T L, Khalling T A, Khristova M L, Allikmets E U, Kharitonenkov I G, Saarma M J, Halonen P
Vopr Virusol. 1991 Jul-Aug;36(4):290-3.
Nine monoclonal antibodies (MCA) to hemagglutinin of influenza A/Taiwan/1/86 (H1N1) virus and 5 MCA to influenza A/Mississippi/1/85 (H3N2) virus were generated and characterized. The MCA were used for the development of diagnostic test systems on the basis of time-resolved fluoroimmunoassay. The same MCA were used as primary and detecting antibodies in the test system specific for HA of the H1 serosubtype, whereas in the test system specific for influenza A serosubtype H3 virus MCA of different epitope appurtenance were used as primary and secondary antibodies. The sensitivity of the test system for HA of serosubtype H1 was found to be 10 ng/ml and that for serosubtype H3 5 nh/ml. The developed test systems were tried on the clinical material collected during the epidemic periods of 1983-1989.
制备并鉴定了9种针对甲型流感病毒/台湾/1/86（H1N1）血凝素的单克隆抗体（MCA）以及5种针对甲型流感病毒/密西西比/1/85（H3N2）的MCA。这些MCA被用于基于时间分辨荧光免疫分析开发诊断测试系统。在针对H1血清亚型HA的测试系统中，相同的MCA被用作一抗和检测抗体，而在针对甲型流感病毒H3血清亚型的测试系统中，不同表位归属的MCA被用作一抗和二抗。发现针对H1血清亚型HA的测试系统灵敏度为10 ng/ml，针对H3血清亚型的为5 ng/ml。所开发的测试系统在1983 - 1989年流行期间收集的临床材料上进行了试验。
