Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea.
Bio-energy Research Institute, Chonnam National University, Gwangju 500-757, Republic of Korea.
Bioresour Technol. 2016 Aug;213:257-261. doi: 10.1016/j.biortech.2016.02.068. Epub 2016 Feb 23.
Jack bean (JB, Canavalia ensiformis) is the source of bio-based products, such as proteins and bio-sugars that contribute to modern molecular biology and biomedical research. In this study, the use of jack bean was evaluated as a source for concanavalin A (Con A) and bio-sugar production. A novel method for purifying Con A from JBs was successfully developed using a glucosylated magnetic nano matrix (GMNM) as a physical support, which facilitated easy separation and purification of Con A. In addition, the enzymatic conversion rate of 2% (w/v) Con A extracted residue to bio-sugar was 98.4%. Therefore, this new approach for the production of Con A and bio-sugar is potentially useful for obtaining bio-based products from jack bean.
杰克豆（JB，Canavalia ensiformis）是生物基产品的来源，如蛋白质和生物糖，这些产品为现代分子生物学和生物医学研究做出了贡献。在这项研究中，评估了杰克豆作为伴刀豆球蛋白 A（Con A）和生物糖生产的来源。使用葡萄糖化磁性纳米基质（GMNM）作为物理支撑，成功开发了一种从 JBs 中纯化 Con A 的新方法，这便于 Con A 的轻松分离和纯化。此外，2%（w/v）Con A 提取残渣转化为生物糖的酶转化率为 98.4%。因此，这种生产 Con A 和生物糖的新方法可能有助于从杰克豆中获得生物基产品。
