Chattaraj Sananda, Purohit Hemant J, Sharma Abhinav, Jadeja Niti B, Madamwar Datta
Environmental Genomics and Proteomics Lab, BRD School of Biosciences, Satellite Campus, Vadtal Road, Sardar Patel University, Vadtal Road, Vallabh Vidyanagar, 388 120, Gujarat, India.
Environmental Genomics Division, CSIR-NEERI, Nehru Marg, Nagpur, 440020, India.
Appl Biochem Biotechnol. 2016 Jun;179(3):514-29. doi: 10.1007/s12010-016-2010-2. Epub 2016 Feb 29.
A laboratory-scale anoxic-oxic sequential reactor system was seeded with acclimatized mixed microbial consortium for the treatment of common effluent treatment plant (CETP) wastewater having 7000-7400 mg L(-1) of COD and 3000-3400 mg L(-1) of BOD. Initially, CETP wastewater was treated under anoxic reactor at 5000 mg L(-1) of MLSS concentrations, 5.26 ± 0.27 kg COD m(-3) day(-1) of organic loading rate (OLR) and 36 h of hydraulic retention time (HRT). Further, the effluent of anoxic reactor was treated in oxic reactor with an OLR of 6.6 ± 0.31 kg COD m(-3) day(-1) and 18 h HRT. Maximum color and COD removal were found to be 72 and 85 % at total HRT of 2.25 days under anoxic-oxic sequential reactor at 37 °C and pH 7.0. The UV-VIS, FTIR, NMR and GCMS studies showed that majority of peaks observed in untreated wastewater were either shifted or disappeared after sequential treatment. Phytotoxicity study with the seeds of Vigna radiata and Triticum aestivum showed more sensitivity toward the CETP wastewater, while the products obtained after sequential treatment does not have any inhibitory effects. The results demonstrated that the anoxic-oxic reactor fed with bacterial consortium VN11 could bring about efficient bioremediation of industrial wastewaters.
一个实验室规模的缺氧-好氧序批式反应器系统接种了经过驯化的混合微生物群落，用于处理化学需氧量（COD）为7000 - 7400 mg L⁻¹、生化需氧量（BOD）为3000 - 3400 mg L⁻¹的普通污水处理厂（CETP）废水。最初，CETP废水在缺氧反应器中于混合液悬浮固体（MLSS）浓度为5000 mg L⁻¹、有机负荷率（OLR）为5.26 ± 0.27 kg COD m⁻³ d⁻¹以及水力停留时间（HRT）为36小时的条件下进行处理。接着，缺氧反应器的出水在好氧反应器中以OLR为6.6 ± 0.31 kg COD m⁻³ d⁻¹和HRT为18小时的条件下进行处理。在37°C和pH 7.0的缺氧-好氧序批式反应器中，总HRT为2.25天时，最大色度和COD去除率分别达到72%和85%。紫外可见光谱（UV-VIS）、傅里叶变换红外光谱（FTIR）、核磁共振（NMR）和气相色谱-质谱联用（GCMS）研究表明，未经处理的废水中观察到的大多数峰在序批处理后要么发生了位移要么消失了。对绿豆和小麦种子进行的植物毒性研究表明，它们对CETP废水更为敏感，而序批处理后获得的产物没有任何抑制作用。结果表明，接种细菌群落VN11的缺氧-好氧反应器能够实现工业废水的高效生物修复。
