Kumar Sushil, Singh Umesh, Deb Rajib, Tyagi Shrikant, Mandal D K, Kumar Mahesh, Sengar G, Sharma Sheetal, Singh Rani, Singh Rupali
Central Institute for Research on Cattle, Indian Council of Agricultural Research, Meerut 250 001, Uttar Pradesh, India.
Meta Gene. 2015 Jul 24;5:140-3. doi: 10.1016/j.mgene.2015.07.004. eCollection 2015 Sep.
The surface expression of CD9 (cluster-of-differentiation antigen-9) in sperms of certain mammalian species has been attributed to its fusion with the egg and thereby dictating the fertility of species. In the present study, we investigated the association of CD9 with crossbred bull sperm quality and quantity trait was analyzed using a total of 96 Frieswal (HF × Sahiwal) crossbred. A single nucleotide polymorphism (g.358A > T) in intron 6 was significantly associated with sperm concentration (P < 0.05) and motility percentage (P < 0.01). mRNA was extracted from good (progressive motility > 50%) and motility impaired (progressive motility < 50%) bull semen. The mRNA expression and seminal plasma protein concentration of CD9 was significantly (P < 0.05) higher among good quality bull semen than motility impaired ones. Our results thus may indicate that, mutation in the intronic region may be responsible for the instability of RNA and the subsequent functional protein expression.
某些哺乳动物精子中CD9（分化簇抗原9）的表面表达被认为与精子和卵子的融合有关，从而决定了物种的生育能力。在本研究中，我们调查了CD9与杂交公牛精子质量的关联，并对总共96头弗里兹瓦尔（HF×萨希瓦尔）杂交公牛的数量性状进行了分析。内含子6中的一个单核苷酸多态性（g.358A>T）与精子浓度（P<0.05）和活力百分比（P<0.01）显著相关。从优质（进行性活力>50%）和活力受损（进行性活力<50%）的公牛精液中提取mRNA。优质公牛精液中CD9的mRNA表达和精浆蛋白浓度显著（P<0.05）高于活力受损的精液。因此，我们的结果可能表明，内含子区域的突变可能导致RNA的不稳定性以及随后的功能性蛋白质表达。
