Valković Toni, Babarović Emina, Lučin Ksenija, Štifter Sanja, Aralica Merica, Seili-Bekafigo Irena, Duletić-Načinović Antica, Jonjić Nives
Department of Hematology, Rijeka University Hospital Centre, Krešimirova 42, 51000 Rijeka, Croatia.
Department of Pathology, School of Medicine, University of Rijeka, Braće Branchetta 20, 51000 Rijeka, Croatia.
Biomed Res Int. 2016;2016:7870590. doi: 10.1155/2016/7870590. Epub 2016 Jan 27.
The aim of this pilot study was to determine the plasma levels of monocyte chemotactic protein-1 (MCP-1) and possible associations with angiogenesis and the main clinical features of untreated patients with multiple myeloma (MM). ELISA was used to determine plasma MCP-1 levels in 45 newly diagnosed MM patients and 24 healthy controls. The blood vessels were highlighted by immunohistochemical staining, and computer-assisted image analysis was used for more objective and accurate determination of two parameters of angiogenesis: microvessel density (MVD) and total vascular area (TVA). The plasma levels of MCP-1 were compared to these parameters and the presence of anemia, renal dysfunction, and bone lesions. A significant positive correlation was found between plasma MCP-1 concentrations and TVA (p = 0.02). The MCP-1 levels were significantly higher in MM patients with evident bone lesions (p = 0.01), renal dysfunction (p = 0.02), or anemia (p = 0.04). Therefore, our preliminary results found a positive association between plasma MCP-1 levels, angiogenesis (expressed as TVA), and clinical features in patients with MM. However, additional prospective studies with a respectable number of patients should be performed to authenticate these results and establish MCP-1 as a possible target of active treatment.
这项初步研究的目的是确定单核细胞趋化蛋白-1（MCP-1）的血浆水平，以及其与血管生成的可能关联和未经治疗的多发性骨髓瘤（MM）患者的主要临床特征。采用酶联免疫吸附测定法（ELISA）测定45例新诊断的MM患者和24例健康对照者的血浆MCP-1水平。通过免疫组织化学染色突出显示血管，并使用计算机辅助图像分析更客观准确地测定血管生成的两个参数：微血管密度（MVD）和总血管面积（TVA）。将MCP-1的血浆水平与这些参数以及贫血、肾功能不全和骨病变的存在情况进行比较。发现血浆MCP-1浓度与TVA之间存在显著正相关（p = 0.02）。在有明显骨病变（p = 0.01）、肾功能不全（p = 0.02）或贫血（p = 0.04）的MM患者中，MCP-1水平显著更高。因此，我们的初步结果发现MM患者的血浆MCP-1水平、血管生成（以TVA表示）与临床特征之间存在正相关。然而，应该进行更多有相当数量患者的前瞻性研究来验证这些结果，并将MCP-1确立为可能的积极治疗靶点。
