Hematology Department, Venizelion Hospital of Heraklion, Knossou Avenue, Zip Code 71409, Heraklion, Greece.
Pathol Oncol Res. 2013 Jul;19(3):539-43. doi: 10.1007/s12253-013-9614-4. Epub 2013 Feb 28.
In multiple myeloma (MM), angiogenesis plays a substantial role in disease progression. Interleukin-8 (IL-8), a pro-inflammatory chemokine with potent pro-angiogenic properties, has been implicated in the pathophysiology of MM. The aim of the study is to measure serum levels of IL-8 in MM patients and to correlate them with markers of angiogenesis, such as circulating levels of platelet derived growth factor-AB (PDGF-AB) and angiogenin (Ang), and bone marrow microvascular density (MVD). Fifty-three newly diagnosed MM patients, 23 of them, who reached plateau phase after effective treatment and 20 healthy controls, were studied. Serum levels of PDGF-AB, Ang and IL-8 were measured by ELISA, whereas bone marrow MVD was estimated by immunohistochemical staining of vessels with anti-CD31. All measured parameters were higher in MM patients compared to controls and in increased disease stages. They all also significantly decreased in plateau phase. IL-8 correlated positively with Ang and PDGF-AB, but not with MVD. The circulating levels of IL-8, PDGF-AB and Ang are elevated in patients with MM. The lack of correlation between IL-8 with MVD suggests that its levels represent the inflammatory element of MM disease and the participation in angiogenesis process is rather complex with multifactorial mechanisms.
在多发性骨髓瘤（MM）中，血管生成在疾病进展中起着重要作用。白细胞介素-8（IL-8）是一种具有强大促血管生成特性的促炎趋化因子，与 MM 的病理生理学有关。本研究旨在测量 MM 患者的血清 IL-8 水平，并将其与血管生成标志物（如循环血小板衍生生长因子-AB（PDGF-AB）和血管生成素（Ang）水平和骨髓微血管密度（MVD）相关联。研究了 53 名新诊断的 MM 患者，其中 23 名在有效治疗后达到平台期，20 名健康对照者。通过 ELISA 测量血清 PDGF-AB、Ang 和 IL-8 水平，通过抗 CD31 免疫组织化学染色测量骨髓 MVD。与对照组相比，MM 患者的所有测量参数均较高，且疾病分期较高。在平台期均显著降低。IL-8 与 Ang 和 PDGF-AB 呈正相关，但与 MVD 无关。MM 患者的循环 IL-8、PDGF-AB 和 Ang 水平升高。IL-8 与 MVD 之间缺乏相关性表明其水平代表 MM 疾病的炎症成分，而参与血管生成过程是相当复杂的，涉及多种机制。
