Yokoyama Yoshihito, Shigeto Tatsuhiko, Miura Rie, Kobayashi Asami, Mizunuma Makito, Yamauchi Aisa, Futagami Masayuki, Mizunuma Hideki
Department of Obstetrics and Gynecology, Hirosaki University Graduate School of Medicine, Hirosaki, Japan E-mail :
Asian Pac J Cancer Prev. 2016;17(2):775-9. doi: 10.7314/apjcp.2016.17.2.775.
The current study examined the effectiveness of concurrent therapy using photodynamic therapy (PDT) and clofibric acid (CA) to treat peritoneal carcinomatosis resulting from ovarian cancer.
Nude rats were used to create a model of peritoneal carcinomatosis resulting from ovarian cancer and the effectiveness of PDT with 5-aminolevulinic acid methyl ester hydrochloride (methyl-ALA-PDT) was determined. The survival time of rats receiving that therapy was compared to the survival time of a control group. Rats with peritoneal carcinomatosis resulting from ovarian cancer were divided into 3 groups: a group that received debulking surgery (DS) alone, a group that received DS+methyl-ALA-PDT, and a group that received DS+methyl-ALA-PDT+CA. The survival time of the 3 groups was compared. Protoporphyrin, a metabolite of methyl-ALA, produces a photochemical action when activated by light. The level of protoporphyrin (the concentration) that reached organs in the abdomen was measured with HPLC.
Rats receiving methyl- ALA-PDT had a significantly longer survival time compared to the controls. Rats with peritoneal carcinomatosis that received DS+methyl-ALA-PDT+CA had a significantly longer survival time compared to the rats that received DS alone. Some of the rats that received concurrent therapy survived for a prolonged period. Protoporphyrin was highly concentrated in peritoneal metastases, but only small amounts reached major organs in the abdomen. PDT was not found to result in necrosis in the intestines.
The results indicated that concurrent therapy consisting of PDT with methyl-ALA and CA is effective at treating peritoneal carcinomatosis resulting from ovarian cancer without damaging organs.
本研究探讨了光动力疗法（PDT）联合氯贝酸（CA）治疗卵巢癌所致腹膜癌的疗效。
使用裸鼠建立卵巢癌所致腹膜癌模型，并确定盐酸5-氨基乙酰丙酸甲酯光动力疗法（甲基-ALA-PDT）的疗效。将接受该治疗的大鼠的存活时间与对照组的存活时间进行比较。将卵巢癌所致腹膜癌的大鼠分为3组：单纯接受肿瘤细胞减灭术（DS）的组、接受DS+甲基-ALA-PDT的组和接受DS+甲基-ALA-PDT+CA的组。比较3组的存活时间。甲基-ALA的代谢产物原卟啉在光照激活时会产生光化学作用。用高效液相色谱法测量到达腹部器官中原卟啉的水平（浓度）。
接受甲基-ALA-PDT的大鼠的存活时间明显长于对照组。与单纯接受DS治疗的大鼠相比，接受DS+甲基-ALA-PDT+CA治疗且患有腹膜癌的大鼠的存活时间明显更长。一些接受联合治疗的大鼠存活时间延长。原卟啉高度集中在腹膜转移灶中，但仅有少量到达腹部主要器官。未发现PDT导致肠道坏死。
结果表明，由甲基-ALA-PDT和CA组成的联合治疗在不损害器官的情况下，对治疗卵巢癌所致腹膜癌有效。
