Neoplasma. 2016;63(3):450-5. doi: 10.4149/315_150930N510.
The presented study aimed to evaluate in vitro the effectiveness of improvement standard chemotherapy with bleomycin by electroporation in two various ovarian cancer cell lines. Two human ovarian cell lines OvBH-1 and SKOV-3 were used. The lines were selected because of their resistance to several therapeutic methods. As anticancer drug we use range of concentrations of bleomycin. In EP and ECT experiments different voltage values: from 0 to 1200 V/cm, 8 pulses with duration of 100μs and intervals between pulses 1s long were used. The cells viability after applied treatments was evaluated by MTT assay. The expression of heat shock proteins - HSP27 was examined by immunocytochemical ABC method.The cytotoxicity with different concentrations of bleomycin alone was not significantly decrease in both cell lines. It confirms resistance of these cells to conventional chemotherapy. The highest decrease of cell proliferation was observed after EP with bleomycin after 48h of incubation for 1000 V/cm. The intensity of expression of small heat shock proteins HSP27 slightly increased after ECT in both treated cell lines, in particular in OvBH-1. The presented study indicated that application of electroporation may effectively enhance chemotherapy with bleomycin, particularly in the case of treating ovarian cancer resistant to standard therapy.
本研究旨在评估电穿孔改良标准化疗博来霉素在两种不同卵巢癌细胞系中的体外疗效。使用了两种人卵巢癌细胞系 OvBH-1 和 SKOV-3。选择这些细胞系是因为它们对多种治疗方法具有耐药性。作为抗癌药物，我们使用了一系列浓度的博来霉素。在 EP 和 ECT 实验中，使用了不同的电压值：从 0 到 1200 V/cm，8 个脉冲，持续时间为 100μs，脉冲间隔为 1s。应用不同浓度博来霉素单独治疗后，通过 MTT 测定评估细胞活力。通过免疫细胞化学 ABC 法检查热休克蛋白 - HSP27 的表达。单独使用不同浓度的博来霉素在两种细胞系中的细胞毒性均未显著降低。这证实了这些细胞对常规化疗的耐药性。在用 1000 V/cm 孵育 48 小时后，EP 联合博来霉素后观察到细胞增殖的最大下降。在用博来霉素进行 ECT 后，两种处理的细胞系中小热休克蛋白 HSP27 的表达强度均略有增加，特别是在 OvBH-1 中。本研究表明，电穿孔的应用可以有效增强博来霉素的化疗效果，特别是在治疗对标准治疗耐药的卵巢癌方面。
