利用具有介电泳作用的光纤尖端对流体中的小颗粒进行灵敏检测。
Sensitive Detection of Small Particles in Fluids Using Optical Fiber Tip with Dielectrophoresis.
作者信息
Tai Yi-Hsin, Chang Dao-Ming, Pan Ming-Yang, Huang Ding-Wei, Wei Pei-Kuen
机构信息
Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan.
Department of Biochemical Science and Technology, National Taiwan University, Taipei 10617, Taiwan.
出版信息
Sensors (Basel). 2016 Feb 27;16(3):303. doi: 10.3390/s16030303.
This work presents using a tapered fiber tip coated with thin metallic film to detect small particles in water with high sensitivity. When an AC voltage applied to the Ti/Al coated fiber tip and indium tin oxide (ITO) substrate, a gradient electric field at the fiber tip induced attractive/repulsive force to suspended small particles due to the frequency-dependent dielectrophoresis (DEP) effect. Such DEP force greatly enhanced the concentration of the small particles near the tip. The increase of the local concentration also increased the scattering of surface plasmon wave near the fiber tip. Combined both DEP effect and scattering optical near-field, we show the detection limit of the concentration for 1.36 μm polystyrene beads can be down to 1 particle/mL. The detection limit of the Escherichia coli (E. coli) bacteria was 20 CFU/mL. The fiber tip sensor takes advantages of ultrasmall volume, label-free and simple detection system.
这项工作展示了使用涂有薄金属膜的锥形光纤尖端来高灵敏度地检测水中的小颗粒。当向涂有钛/铝的光纤尖端和氧化铟锡（ITO）基板施加交流电压时，由于频率依赖的介电泳（DEP）效应，光纤尖端处的梯度电场会对悬浮的小颗粒产生吸引/排斥力。这种DEP力极大地提高了尖端附近小颗粒的浓度。局部浓度的增加也增加了光纤尖端附近表面等离子体波的散射。结合DEP效应和散射光学近场，我们表明对于1.36μm聚苯乙烯珠，浓度检测限可低至1个颗粒/毫升。大肠杆菌（E. coli）细菌的检测限为20 CFU/毫升。光纤尖端传感器具有超小体积、无需标记和检测系统简单的优点。
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