Tawil N, Sacher E, Mandeville R, Meunier M
Regroupement Québécois de Matériaux de Pointe, Department of Engineering Physics, École Polytechnique de Montréal, Case Postale 6079, succursale Centre-ville, Montréal, Québec H3C 3A7, Canada.
Analyst. 2014 Mar 21;139(6):1224-36. doi: 10.1039/c3an01989f.
Pathogen detection is of utmost importance in many sectors, such as in the food industry, environmental quality control, clinical diagnostics, bio-defence and counter-terrorism. Failure to appropriately, and specifically, detect pathogenic bacteria can lead to serious consequences, and may ultimately be lethal. Public safety, new legislation, recent outbreaks in food contamination, and the ever-increasing prevalence of multidrug-resistant infections have fostered a worldwide research effort targeting novel biosensing strategies. This review concerns phage-based analytical and biosensing methods targeted towards theranostic applications. We discuss and review phage-based assays, notably phage amplification, reporter phage, phage lysis, and bioluminescence assays for the detection of bacterial species, as well as phage-based biosensors, including optical (comprising SPR sensors and fiber optic assays), electrochemical (comprising amperometric, potentiometric, and impedimetric sensors), acoustic wave and magnetoelastic sensors.
病原体检测在许多领域都至关重要，如食品工业、环境质量控制、临床诊断、生物防御和反恐等。未能适当地、特异性地检测出病原菌可能会导致严重后果，甚至最终可能致命。公共安全、新法规、近期的食品污染事件以及多重耐药感染的日益流行，推动了全球针对新型生物传感策略的研究工作。本综述涉及用于治疗诊断应用的基于噬菌体的分析和生物传感方法。我们讨论并综述了基于噬菌体的检测方法，特别是用于检测细菌种类的噬菌体扩增、报告噬菌体、噬菌体裂解和生物发光检测，以及基于噬菌体的生物传感器，包括光学传感器（包括表面等离子体共振传感器和光纤检测）、电化学传感器（包括安培型、电位型和阻抗型传感器）、声波传感器和磁弹性传感器。
