Devost Dominic, Audet Nicolas, Zhou Cynthia, Kobayashi Hiroyuki, Bonin Hélène, Lukashova Viktorya, Le Gouill Christian, Bouvier Michel, Hébert Terence E
Department of Pharmacology and Therapeutics, McGill University, Montréal, QC, Canada.
Department of Biochemistry and Molecular Medicine, Institute for Research in Immunology and Cancer, Université de Montréal, Montreal, QC, Canada.
Methods Cell Biol. 2016;132:319-37. doi: 10.1016/bs.mcb.2015.11.010. Epub 2016 Jan 29.
The use of biosensors either individually or as part of panels has now become a common technique to capturing signaling events in living cells. Such biosensors have become particularly important for probing biased signaling and allostery in G protein-coupled receptor drug screening efforts. However, assumptions about the portability of such biosensors between cell types may lead to misinterpretation of drug effects on specific signaling pathways in a given cellular context. Further, the output of a particular biosensor may be different depending on where it is localized in a cell. Here, we discuss strategies to mitigate these concerns which should feed into future biosensor design and usage.
单独使用生物传感器或将其作为检测板的一部分，如今已成为捕获活细胞中信号事件的常用技术。此类生物传感器在G蛋白偶联受体药物筛选工作中探测偏向性信号传导和变构作用时已变得尤为重要。然而，关于此类生物传感器在不同细胞类型之间可移植性的假设，可能会导致在特定细胞环境中对药物对特定信号通路的影响产生误解。此外，特定生物传感器的输出可能因其在细胞中的定位不同而有所差异。在此，我们讨论可减轻这些问题的策略，这些策略应会影响未来生物传感器的设计和使用。
