颗粒酶M和K在人类实验性内毒素血症中的释放。
Granzyme M and K release in human experimental endotoxemia.
作者信息
Wensink Annette C, Wiewel Maryse A, Jongeneel Lieneke H, Boes Marianne, van der Poll Tom, Hack C Erik, Bovenschen Niels
机构信息
Department of Pathology, University Medical Center Utrecht, Utrecht 3584 CX, The Netherlands; Laboratory of Translational Immunology, University Medical Center Utrecht, Utrecht 3584 CX, The Netherlands.
Center for Experimental and Molecular Medicine, Amsterdam Medical Center, University of Amsterdam, Amsterdam 1100 DD, The Netherlands.
出版信息
Immunobiology. 2016 Jul;221(7):773-7. doi: 10.1016/j.imbio.2016.02.006. Epub 2016 Feb 24.
Granzymes are serine proteases involved in killing of tumor cells and virally infected cells. However, granzymes are also upregulated in blood under inflammatory conditions and contribute to cytokine release and processing. Here, we show that granzyme M (GrM) and to a lesser extent GrK are transiently elevated in the circulation following LPS administration in humans. GrM is released upon stimulation of whole blood with LPS or the gram-negative bacteria Escherichia coli BL21, Pseudomonas aeruginosa, and Neisseria meningitidis. GrK is only released upon stimulation with P. aeruginosa. Thus, GrM and GrK are differentially released in response to LPS and gram-negative bacteria.
颗粒酶是参与杀伤肿瘤细胞和病毒感染细胞的丝氨酸蛋白酶。然而，在炎症条件下，颗粒酶在血液中的表达也会上调，并促进细胞因子的释放和加工。在此，我们表明，在人类中给予脂多糖（LPS）后，颗粒酶M（GrM）以及程度较轻的颗粒酶K（GrK）在循环系统中会短暂升高。用LPS或革兰氏阴性菌大肠杆菌BL21、铜绿假单胞菌和脑膜炎奈瑟菌刺激全血时，GrM会释放出来。GrK仅在受到铜绿假单胞菌刺激时才会释放。因此，GrM和GrK对LPS和革兰氏阴性菌的反应是差异释放的。
Zotero 插件