Langenheder Silke, Comte Jérôme, Zha Yinghua, Samad Md Sainur, Sinclair Lucas, Eiler Alexander, Lindström Eva S
Department of Ecology and Genetics/Limnology, Uppsala University, Norbyvägen 18D, 75236, Uppsala, Sweden.
Department of Microbiology and Immunology, University of Otago, 720 Cumberland St. North Dunedin, 9016, Dunedin, New Zealand.
Environ Microbiol Rep. 2016 Aug;8(4):479-85. doi: 10.1111/1758-2229.12392. Epub 2016 May 11.
Some bacteria can be preserved over time in deep sediments where they persist either in dormant or slow-growing vegetative stages. Here, we hypothesized that such cells can be revived when exposed to environmental conditions similar to those before they were buried in the sediments. To test this hypothesis, we collected bacteria from sediment samples of different ages (140-8500 calibrated years before present, cal BP) from three lakes that differed in the timing of their physical isolation from the Baltic Sea following postglacial uplift. After these bacterial communities were grown in sterile water from the Baltic Sea, we determined the proportion of 16S rRNA sequence reads associated with marine habitats by extracting the environment descriptive terms of homologous sequences retrieved from public databases. We found that the proportion of reads associated with marine descriptive term was significantly higher in cultures inoculated with sediment layers formed under Baltic conditions and where salinities were expected to be similar to current levels. Moreover, a similar pattern was found in the original sediment layers. Our study, therefore, suggests that remnants of marine bacterial communities can be preserved in sediments over thousands of years and can be revived from deep sediments in lakes of marine origin.
一些细菌可以长时间保存在深层沉积物中，它们在那里以休眠或生长缓慢的营养阶段持续存在。在这里，我们假设当暴露于与它们被埋入沉积物之前相似的环境条件时，这些细胞可以复苏。为了验证这一假设，我们从三个湖泊不同年代（距今140 - 8500校准年，cal BP）的沉积物样本中收集细菌，这三个湖泊在冰后期隆升后与波罗的海物理隔离的时间不同。在这些细菌群落于波罗的海无菌水中培养后，我们通过提取从公共数据库检索到的同源序列的环境描述性术语，确定了与海洋栖息地相关的16S rRNA序列读数的比例。我们发现，接种了在波罗的海条件下形成的沉积层且预计盐度与当前水平相似的培养物中，与海洋描述性术语相关的读数比例显著更高。此外，在原始沉积层中也发现了类似的模式。因此，我们的研究表明，海洋细菌群落的残余物可以在沉积物中保存数千年，并且可以从海洋起源的湖泊深层沉积物中复苏。
