Song Naining, Li Lei, Li Haishan, Ai Wenchao, Xie Wenping, Yu Wenlian, Liu Wei, Wang Cheng, Shen Guolin, Zhou Lili, Wei Changlei, Li Dong, Chen Huiming
Institute of Chemicals Safety, Chinese Academy of Inspection and Quarantine, No. TA11 Shuangqiao Middle Road, Chaoyang District, Beijing 100121, PR China.
Institute of Chemicals Safety, Chinese Academy of Inspection and Quarantine, No. TA11 Shuangqiao Middle Road, Chaoyang District, Beijing 100121, PR China.
Food Chem Toxicol. 2016 May;91:73-81. doi: 10.1016/j.fct.2016.02.020. Epub 2016 Feb 27.
Limited toxicological information is available for hexabromocyclododecane (HBCD),a widely used additive brominated flame retardant. Inhalation is a major route of human exposure to HBCD. The aim of this study was to determine the acute inhalation toxicity and potential subchronic inhalation toxicity of HBCD in Sprague-Dawley rats exposed to HBCD only through inhalation. The acute inhalation toxicity of HBCD was determined using the limit test method on five male and five female Sprague-Dawley rats at a HBCD concentration of 5000 mg/m(3). Repeated-dose toxicity tests were also performed, with 20 males and 20 females randomly assigned to four experimental groups (five rats of each sex in each group). There were three treatment groups (exposed to HBCD concentrations of 125,500, and 2000 mg/m(3)) and a blank control group (exposed to fresh air). In the acute inhalation toxicity study, no significant clinical signs were observed either immediately after exposure or during the recovery period. Gross pathology examination revealed no evidence of organ-specific toxicity in any rat. The inhalation LC50(4 h) for HBCD was higher than 5312 ± 278 mg/m3 for both males and females. In the repeated dose inhalation study, daily head/nose-only exposure to HBCD at 132 ± 8.8, 545.8 ± 35.3, and 2166.0 ± 235.9 mg/m(3) for 14 days caused no adverse effects. No treatment-related clinical signs were observed at any of the test doses. The NOAEL for 14-day repeated dose inhalation toxicity study of HBCD is 2000 mg/m(3).
六溴环十二烷（HBCD）是一种广泛使用的添加型溴化阻燃剂，目前关于它的毒理学信息有限。吸入是人类接触HBCD的主要途径。本研究的目的是确定仅通过吸入接触HBCD的斯普拉格-道利大鼠中HBCD的急性吸入毒性和潜在的亚慢性吸入毒性。采用极限试验法，对5只雄性和5只雌性斯普拉格-道利大鼠进行HBCD急性吸入毒性测定，HBCD浓度为5000mg/m³。还进行了重复剂量毒性试验，将20只雄性和20只雌性大鼠随机分为4个实验组（每组每种性别各5只大鼠）。有3个治疗组（分别暴露于浓度为125、500和2000mg/m³的HBCD）和1个空白对照组（暴露于新鲜空气）。在急性吸入毒性研究中，暴露后立即或恢复期均未观察到明显的临床症状。大体病理学检查未发现任何大鼠有器官特异性毒性的证据。HBCD对雄性和雌性大鼠的吸入LC50（4小时）均高于5312±278mg/m³。在重复剂量吸入研究中，每天仅头部/鼻子暴露于浓度为132±8.8、545.8±35.3和2166.0±235.9mg/m³的HBCD中14天，未产生不良影响。在任何试验剂量下均未观察到与治疗相关的临床症状。HBCD 14天重复剂量吸入毒性研究的无观察到有害作用水平（NOAEL）为2000mg/m³。
