Kim Sung Min, Lim Kyung Tae, Kwak Tae Hwan, Lee Seung Chan, Im Jung Hyun, Hali Sai, In Hwang Seon, Kim Dajeong, Hwang Jeongho, Kim Kee-Pyo, Chung Hak-Jae, Kim Jeong Beom, Ko Kinarm, Chung Hyung-Min, Lee Hoon Taek, Schöler Hans R, Han Dong Wook
Department of Stem Cell Biology, School of Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea; Department of Animal Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
Department of Stem Cell Biology, School of Medicine, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
Stem Cell Res. 2016 Mar;16(2):460-8. doi: 10.1016/j.scr.2016.02.025. Epub 2016 Feb 10.
Somatic cells could be directly converted into induced neural stem cells (iNSCs) by ectopic expression of defined transcription factors. However, the underlying mechanism of direct lineage transition into iNSCs is largely unknown. In this study, we examined the effect of genetic background on the direct conversion process into an iNSC state. The iNSCs from two different mouse strains exhibited the distinct efficiency of lineage conversion as well as clonal expansion. Furthermore, the expression levels of endogenous NSC markers, silencing of transgenes, and in vitro differentiation potential were also different between iNSC lines from different strains. Therefore, our data suggest that the genetic background of starting cells influences the conversion efficiency as well as reprogramming status of directly converted iNSCs.
通过异位表达特定转录因子，体细胞可直接转化为诱导神经干细胞（iNSCs）。然而，直接谱系转变为iNSCs的潜在机制在很大程度上尚不清楚。在本研究中，我们研究了遗传背景对直接转化为iNSC状态过程的影响。来自两种不同小鼠品系的iNSCs表现出不同的谱系转化效率以及克隆扩增能力。此外，不同品系的iNSC系之间，内源性NSC标志物的表达水平、转基因的沉默以及体外分化潜能也存在差异。因此，我们的数据表明起始细胞的遗传背景会影响直接转化的iNSCs的转化效率以及重编程状态。
