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[2014年郴州市发热患者疟疾血涂片制备的质量分析]

[Quality analysis of malaria blood smear preparations of febrile patients in Chenzhou Prefecture, 2014].

作者信息

Wang Yan-qin, Zhu Han-wu

出版信息

Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2015 Oct;27(5):549-50, 554.

Abstract

OBJECTIVE

To standardize the malaria smear preparations of febrile patients in Chenzhou Prefecture, Hunan Province, so as to provide the technical support for malaria elimination.

METHODS

According to the Technical Solutions to Eliminate Malaria (2011 edition) , the blood smear preparations of febrile patients from each county, included more than 3% negatives and all positives, were reviewed monthly in 2014. The quality of blood smear manufacture, dyeing, cleanliness and results was reviewed by malaria microscopic examination experts. The data were analyzed with the descriptive epidemiological methods.

RESULTS

Totally 231 blood smears were reviewed in 2014 with a reviewed rate of 6.91%. The blood smear production qualified rate was 80.52%, the dyeing pass rate was 84.42%, the cleanliness pass rate was 86.58%, and there were no false detections and no leak detections. The highest blood smear production qualified rate, dyeing pass rate and cleanliness pass rate were found in Guiyang County and Linwu County, with all the rates of 100% respectively. The lowest blood smear production qualified rate and cleanliness pass rate were found in Yizhang County, with the rates of 52.94 % and 70.59% respectively. The lowest blood smear dyeing pass rate was found in Yongxing County with the rate of 63.64%. There were statistically significant differences between Guiyang County and Yizhang County in the production qualified rates and cleanliness pass rates (χ2 = 18.60, 9.73, both P < 0.01). There was a statistically significant difference between Guiyang County and Yongxing County in the dyeing pass rates (χ2 = 11.43, P < 0.01).

CONCLUSION

Through the review of blood smears, the problems will be timely discovered. Therefore, the blood smear quality should improve, which is helpful for achieving the goal of malaria elimination.

摘要

目的

规范湖南省郴州市发热病人疟原虫血片制作,为消除疟疾提供技术支持。

方法

按照《消除疟疾技术方案(2011年版)》要求,2014年每月对各县超过3%的阴性及全部阳性发热病人血片制作情况进行审核,由疟疾镜检专家对血片制作、染色、清洁度及结果质量进行审核,采用描述性流行病学方法进行数据分析。

结果

2014年共审核血片231张,审核率为6.91%。血片制作合格率为80.52%,染色合格率为84.42%,清洁度合格率为86.58%,无漏检和误检情况。桂阳县和临武县血片制作合格率、染色合格率和清洁度合格率均最高,均为100%。宜章县血片制作合格率和清洁度合格率最低,分别为52.94%和70.59%。永兴县血片染色合格率最低,为63.64%。桂阳县与宜章县血片制作合格率和清洁度合格率差异有统计学意义(χ2 = 18.60、9.73,P均<0.01)。桂阳县与永兴县血片染色合格率差异有统计学意义(χ2 = 11.43,P < 0.01)。

结论

通过血片审核能及时发现问题,有助于提高血片质量,对实现消除疟疾目标有促进作用。

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