不同培养条件对高档韩牛卵巢来源胚胎体外受精后卵母细胞及胚胎发育的影响。

Effects of culture media conditions on production of eggs fertilized in vitro of embryos derived from ovary of high grade Hanwoo.

机构信息

Department of Animal Resources, Daegu University, Gyeongbuk, 38453 Korea.

Agricultural Technology Center, Gyeongbuk, 39687 Korea.

出版信息

J Anim Sci Technol. 2016 Mar 1;58:11. doi: 10.1186/s40781-016-0093-5. eCollection 2016.

DOI:10.1186/s40781-016-0093-5

PMID:26933508

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4772279/

Abstract

BACKGROUND

This study was investigated the effects of culture media conditions on production of eggs fertilized in vitro of embryos from ovaries of high grade Korean native cow, Hanwoo.

METHODS

The IVMD 101 and IVF 100 were used for in vitro maturation of selected Hanwoo oocytes and In vitro embryo culture after in vitro fertilization, respectively. The IVMD 101 and IVD 101 were used for in vitro culture and completely free of serum.

RESULTS

The cleavage rates of 2-cell embryos in reference to Hanwoo oocytes were 86.7, 92.9 , and 90.1 % in the control group, IVDM101 medium and IVD101 medium, respectively which indicates that the IVDM101 medium and IVD101 medium may result favorable outcomes. The in vitro development rates of blastocysts were 12.4, 38.4 and 32.4 % in the control group, serum free IVMD101 medium and IVD101 medium, respectively. For hatched blastocysts, it was 5.3, 33.9, and 28.6 % in the control group, serum free IVMD101 medium and IVD101 medium, respectively. Hence, more favorable results were expected for the hatched blastocysts in which the IVMD101 medium and IVD101 medium were used than the control group. Average cell numbers of blastocysts were 128.3, 165.7, and 163.6 in the groups of TCM-199 + 10 % FBS medium, IVMD 101 medium, and IVD 101 medium, respectively which clearly show that the IVMD 101 and IVD 101 medium consequence significantly higher cell numbers compared to the control group (i.e., TCM-199 + 10 % FBS medium). Pregnancy rate after embryo transfer was 39.6 % when the serum free medium was used which is higher than that of the medium supplemented with serum (32.8 %). In addition, stillbirth rates were 4.9 % in the group of serum free medium whereas it was 13.6 % in the serum supplemented medium (13.6 %).

CONCLUSIONS

Taken altogether, serum free media, the IVMD 101 and IVD 101 represented more favorable results in the embryo development rate of embryos, cell numbers of blastocyst, and pregnancy rate. Of note, the IVMD 101 medium showed better outcomes hence, it might be a better option for future applications for in vitro culture of bovine embryos.

摘要

背景

本研究旨在探讨不同培养基条件对韩国高档韩牛（Hanwoo）卵巢中体外受精胚胎的卵母细胞体外成熟、体外受精后胚胎培养的影响。

方法

本研究使用 IVMD101 和 IVF100 分别进行选择的 Hanwoo 卵母细胞的体外成熟和体外受精后的体外胚胎培养。IVMD101 和 IVD101 用于体外培养，完全不含血清。

结果

参照 Hanwoo 卵母细胞，对照组、IVDM101 培养基和 IVD101 培养基中 2 细胞胚胎的卵裂率分别为 86.7%、92.9%和 90.1%，这表明 IVDM101 培养基和 IVD101 培养基可能会产生有利的结果。囊胚的体外发育率分别为对照组、无血清 IVMD101 培养基和 IVD101 培养基中的 12.4%、38.4%和 32.4%。对于孵育囊胚，对照组、无血清 IVMD101 培养基和 IVD101 培养基中的比例分别为 5.3%、33.9%和 28.6%。因此，与对照组相比，使用 IVMD101 培养基和 IVD101 培养基的孵育囊胚可能会产生更好的结果。囊胚的平均细胞数分别为 TCM-199+10%FBS 培养基组 128.3、IVMD101 培养基组 165.7 和 IVD101 培养基组 163.6，这清楚地表明 IVMD101 和 IVD101 培养基的细胞数明显高于对照组（即 TCM-199+10%FBS 培养基）。胚胎移植后的妊娠率为 39.6%，使用无血清培养基时高于添加血清的培养基（32.8%）。此外，无血清培养基组的死胎率为 4.9%，而添加血清的培养基组为 13.6%（13.6%）。