Bortoli S A De, Vacari A M, Laurentis V L, Bortoli C P De, Santos R F, Otuka A K
Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, SP, Brazil.
Braz J Biol. 2016 Jun;76(2):307-14. doi: 10.1590/1519-6984.12914. Epub 2016 Mar 1.
Mass production of predatory stinkbugs in the laboratory is prioritized to release them into the field as part of IPM programs. Therefore, the aim of this study was to identify the best prey for rearing the predator Podisus nigrispinus (Dallas, 1851) (Hemiptera: Pentatomidae) among five different species of insect (three of Lepidoptera, one of Coleoptera, and one of Diptera). Second-instar P. nigrispinus nymphs were conditioned in transparent 1000-mL plastic pots, adults were placed in Petri dishes for mating, and both stages were maintained under controlled conditions (25 ± 1°C, 12 hours of photophase, 70 ± 10% RH). Nymphs and adults of P. nigrispinus consumed more Musca domestica (Linnaeus, 1758) (Diptera: Muscidae) larvae than the other tested prey. The consumption of fly larvae was 1.5 larvae/day/nymph and adults 1.7 larvae/day/adult. However, the number of eggs per female was less when the predator consumed M. domestica larvae (407.8 eggs/female) and most when consumed the Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae) larvae (797.7 eggs/female). Furthermore, the percentage of hatched eggs was greater when the predator females consumed D. saccharalis larvae (90.0%). D. saccharalis larvae is the best prey to rearing P. nigrispinus.
在实验室中大规模生产捕食性椿象被列为优先事项，以便将其作为综合虫害管理计划的一部分释放到田间。因此，本研究的目的是在五种不同昆虫物种（三种鳞翅目、一种鞘翅目和一种双翅目）中，确定用于饲养捕食者黑角红蝽（Podisus nigrispinus (Dallas, 1851)，半翅目：蝽科）的最佳猎物。黑角红蝽二龄若虫饲养在透明的1000毫升塑料盆中，成虫放置在培养皿中进行交配，两个阶段均保持在受控条件下（25±1°C，光照期12小时，相对湿度70±10%）。与其他测试猎物相比，黑角红蝽的若虫和成虫消耗的家蝇（Musca domestica (Linnaeus, 1758)，双翅目：蝇科）幼虫更多。若虫每天消耗1.5只蝇幼虫/若虫，成虫每天消耗1.7只蝇幼虫/成虫。然而，当捕食者消耗家蝇幼虫时，每只雌虫的产卵数较少（407.8枚卵/雌虫），而当消耗甘蔗二点螟（Diatraea saccharalis (Fabricius, 1794)，鳞翅目：草螟科）幼虫时产卵数最多（797.7枚卵/雌虫）。此外，当捕食者雌虫消耗甘蔗二点螟幼虫时，孵化卵的百分比更高（90.0%）。甘蔗二点螟幼虫是饲养黑角红蝽的最佳猎物。
