L-精氨酸是鲑鱼精巢核蛋白的一种活性成分，它通过秀丽隐杆线虫中的沉默信息调节因子促进耐热性。
L-arginine, an active component of salmon milt nucleoprotein, promotes thermotolerance via Sirtuin in Caenorhabditis elegans.
作者信息
Furuhashi Tsubasa, Matsunaga Masaji, Asahara Yuji, Sakamoto Kazuichi
机构信息
Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8572, Japan.
Gene Trophology Institute Co., 3-1-3 Megumino Kita, Eniwa, Hokkaido 061-1374, Japan.
出版信息
Biochem Biophys Res Commun. 2016 Mar 25;472(1):287-91. doi: 10.1016/j.bbrc.2016.02.114. Epub 2016 Mar 2.
We previously showed that salmon milt nucleoprotein (NP) promotes thermotolerance in Caenorhabditis elegans; however, the active component and physiological mechanism of this effect has remained unclear. l-arginine (AR) is a major component of protamine and thus it has been proposed as the possible active component of NP. In this study, the viability of C. elegans treated with AR under heat stress was assessed and AR was shown to extend the survival term of the heat-stressed organisms. Additionally, AR was shown to restore the thrashing movement of the worms that is suppressed by heat stress. Treatment with AR was furthermore shown to promote thermotolerance in a DAF-16- and SIR-2.1-dependent manner, where DAF-16 and SIR-2.1 are homologs of FoxO and SirT1, respectively. Taken together, these data suggest that AR is one of the active components of NP and promotes thermotolerance via the activation of DAF-16 and SIR-2.1.
我们之前表明，鲑鱼精巢核蛋白（NP）可促进秀丽隐杆线虫的耐热性；然而，这种效应的活性成分和生理机制仍不清楚。L-精氨酸（AR）是鱼精蛋白的主要成分，因此有人提出它可能是NP的活性成分。在本研究中，评估了热应激下用AR处理的秀丽隐杆线虫的活力，结果显示AR可延长热应激生物体的存活期。此外，AR还可恢复受热应激抑制的线虫的摆动运动。进一步研究表明，AR以依赖DAF-16和SIR-2.1的方式促进耐热性，其中DAF-16和SIR-2.1分别是FoxO和SirT1的同源物。综上所述，这些数据表明AR是NP的活性成分之一，并通过激活DAF-16和SIR-2.1促进耐热性。
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