Zasowska-Nowak Anna, Nowak Piotr J, Bialasiewicz Piotr, Prymont-Przyminska Anna, Zwolinska Anna, Sarniak Agata, Wlodarczyk Anna, Markowski Jaroslaw, Rutkowski Krzysztof P, Nowak Dariusz
a Palliative Medicine Laboratory.
b Department of Nephrology , Hypertension and Kidney Transplantation.
J Am Coll Nutr. 2016 Jul;35(5):422-35. doi: 10.1080/07315724.2015.1065523. Epub 2016 Mar 2.
Strawberries can improve oxidants-antioxidants balance and reduce some cardiovascular risk factors in obese subjects. Paraoxonase-1 (PON-1) is a high-density lipoprotein-associated enzyme with antioxidant properties that can protect from coronary artery disease in humans. We examined the effect of strawberry consumption on plasma PON-1 activity and lipid profile in healthy nonobese subjects.
Thirty-one subjects (body mass index [BMI] 24.4 ± 4.0 kg/m(2)) on their usual diet consumed 500 g of strawberry pulp daily for 30 days (first course) and after a 10-day washout the cycle was repeated (second course). Fasting blood and spot morning urine samples were collected before, during, and after each strawberry course (8 time points) for determination of paraoxonase and arylesterase PON-1 activities and lipid profile. Twenty subjects served as controls with respect to cholesterol and PON-1 activities changes over the study period.
Strawberries decreased mean plasma paraoxonase PON-1 activity and this effect was more evident after the second course (by 11.6%, p < 0.05) than after the first course (5.4%, p = 0.06), whereas arylesterase activity was constant. Strawberries altered total cholesterol levels (p < 0.05) with a tendency to transiently decrease it (by 5.1%) only after 15 days of the first course. Triglycerides and high- and low-density lipoprotein cholesterol did not change in response to fruit consumption. No changes in PON-1 activities and lipid profile were noted in controls. Paraoxonase correlated with arylesterase activity (ƿ from 0.33 to 0.46 at the first 7 time points, p < 0.05). This association disappeared at the end of study (ƿ = 0.07) when the strongest inhibition of paraoxonase was noted.
Supplementation of the usual diet with strawberries decreased paraoxonase PON-1 activity and did not improve lipid profiles in healthy nonobese subjects. Further studies are necessary to establish the clinical significance of paraoxonase suppression and to define a group of healthy subjects who can benefit from strawberry consumption with respect to cholesterol levels.
草莓可改善肥胖受试者体内氧化剂 - 抗氧化剂平衡，并降低一些心血管危险因素。对氧磷酶 -1（PON -1）是一种与高密度脂蛋白相关的具有抗氧化特性的酶，可保护人类免受冠状动脉疾病的侵害。我们研究了食用草莓对健康非肥胖受试者血浆PON -1活性和血脂谱的影响。
31名受试者（体重指数[BMI]为24.4±4.0kg/m²）按日常饮食，每天食用500克草莓果肉，持续30天（第一疗程），在10天的洗脱期后重复该周期（第二疗程）。在每个草莓疗程之前、期间和之后（共8个时间点）采集空腹血液和晨尿样本，以测定对氧磷酶和芳基酯酶的PON -1活性以及血脂谱。20名受试者作为对照，观察研究期间胆固醇和PON -1活性的变化。
草莓降低了血浆对氧磷酶PON -1的平均活性，且这种作用在第二疗程后（降低11.6%，p<0.05）比第一疗程后（降低5.4%，p = 0.06）更明显，而芳基酯酶活性保持不变。草莓改变了总胆固醇水平（p<0.05），仅在第一疗程15天后有短暂降低的趋势（降低5.1%）。甘油三酯以及高密度和低密度脂蛋白胆固醇水平未因食用水果而发生变化。对照组的PON -1活性和血脂谱无变化。在前7个时间点，对氧磷酶与芳基酯酶活性相关（r值从0.33至0.46，p<0.05）。当观察到对氧磷酶受到最强抑制时，这种关联在研究结束时消失（r = 0.07）。
在健康非肥胖受试者的日常饮食中添加草莓会降低对氧磷酶PON -1活性，且未改善血脂谱。有必要进一步研究以确定对氧磷酶抑制的临床意义，并确定一组能从食用草莓中在胆固醇水平方面获益的健康受试者群体。
