Zheng Jin, Dong Weijiang, Zhang Jiangwei, Li Guangyue, Gong Huilin
Department of Kidney Transplant, Hospital of Nephrology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
Department of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China.
Acta Biochim Biophys Sin (Shanghai). 2016 Apr;48(4):318-25. doi: 10.1093/abbs/gmw012. Epub 2016 Mar 1.
Y box protein 1 (YB-1) is a multifunctional cellular protein expressed in various cancers, and is a potential target in cancer therapy. Although there is evidence showing that YB-1 plays a role in human cancers, the clinical significance of YB-1 expression in glioma has not been established. In the present study, we investigated the YB-1 level in glioma tumors and analyzed the relationship between the YB-1 level and the grade of malignant glioma, with the aim of providing new ideas for the diagnosis and treatment of gliomas in clinical and basic research settings. A total of 108 patients, comprising 14, 31, 30, and 33 with gliomas of Grades I, II, III, and IV, respectively, were included in this study. The mRNA and protein levels of YB-1 were found to be significantly different between Grade IV and lower-grade tumors. The YB-1 levels in cerebrospinal fluid were significantly higher in Grades III and IV glioma patients than in Grades I and II patients. Immunofluorescence staining was used to detect the levels of YB-1 in the cytoplasm and the nucleus, and results indicated that the intracellular distribution was significantly associated with the pathological grade of glioma. A higher level of YB-1 was associated with shortened survival, suggesting that YB-1 plays a role in the progression of human glioma.
Y盒蛋白1（YB-1）是一种在多种癌症中表达的多功能细胞蛋白，是癌症治疗中的一个潜在靶点。尽管有证据表明YB-1在人类癌症中发挥作用，但YB-1在胶质瘤中的表达的临床意义尚未明确。在本研究中，我们调查了胶质瘤肿瘤中的YB-1水平，并分析了YB-1水平与恶性胶质瘤分级之间的关系，旨在为临床和基础研究中胶质瘤的诊断和治疗提供新思路。本研究共纳入108例患者，分别为14例、31例、30例和33例I级、II级、III级和IV级胶质瘤患者。发现IV级肿瘤与低级别肿瘤之间YB-1的mRNA和蛋白水平存在显著差异。III级和IV级胶质瘤患者脑脊液中的YB-1水平显著高于I级和II级患者。采用免疫荧光染色检测细胞质和细胞核中YB-1的水平，结果表明细胞内分布与胶质瘤的病理分级显著相关。较高水平的YB-1与生存期缩短相关，提示YB-1在人类胶质瘤进展中起作用。
