Oda Yoshimi, Nakashima Souichi, Nakamura Seikou, Yano Mamiko, Akiyama Masanori, Imai Kayo, Kimura Tomohito, Nakata Akiko, Tani Miyuki, Matsuda Hisashi
Department of Pharmacognosy, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto, 607-8412, Japan.
N. T. H Co., Ltd., 1-8-11 4F Sky-ebisu Bldg., Ebisu, Shibuya-ku, Tokyo, 150-0013, Japan.
J Nat Med. 2016 Jul;70(3):384-90. doi: 10.1007/s11418-016-0974-4. Epub 2016 Mar 2.
The methanolic extract of Lawsonia inermis L. (henna) showed accelerative effects on nerve growth factor-induced neurite outgrowth in PC12 cells under non-fasting conditions. To elucidate the active constituents responsible for the neuronal differentiation, we conducted a search of the constituents and examined their accelerative effects on neurite outgrowth in PC12 cells. We isolated a new acetophenone glycoside, inermioside A, which exerted a significant accelerative effect on neurite outgrowth. We also confirmed the activities of nine known compounds, including quercetin and lalioside. In addition, we found that quercetin, one of the active constituents, increased Vav3 mRNA expression.
无患子（散沫花）的甲醇提取物在非饥饿条件下对神经生长因子诱导的PC12细胞神经突生长具有促进作用。为阐明负责神经元分化的活性成分成分，我们对其成分进行了筛选，并检测了它们对PC12细胞神经突生长的促进作用。我们分离出一种新的苯乙酮糖苷——无患子苷A，它对神经突生长具有显著的促进作用。我们还证实了包括槲皮素和无患子苷在内的9种已知化合物的活性。此外，我们发现活性成分之一的槲皮素可增加Vav3 mRNA的表达。
