Bambra C S, Isahakia M
Institute of Primate Research, National Museums of Kenya Karen, Nairobi.
J Reprod Immunol. 1989 Dec;16(3):207-16. doi: 10.1016/0165-0378(89)90050-8.
Three monoclonal antibodies (BM2, BM3 and BM4) were obtained from two hybridomas produced after murine immunization with total placental cell dispersion obtained from animals at day 33 of gestation. Immunohistochemical studies showed that antigens recognised by these three antibodies were located on villous trophoblast of fixed placental sections at day 33 of pregnancy. Other fixed normal baboon tissues tested were non-reactive with these antibodies. Monoclonal antibody BM2 was found to stain villous cytotrophoblast; BM3 and BM4 reacted with syncytiotrophoblast but with different staining characteristics. Immunoblot studies showed that BM2, BM3 and BM4 antibodies recognized antigens corresponding to 30.9, 31.3 and 42.3 kDa, respectively.
用妊娠第33天动物的胎盘全细胞匀浆免疫小鼠后产生的两个杂交瘤，获得了三种单克隆抗体（BM2、BM3和BM4）。免疫组织化学研究表明，这三种抗体识别的抗原位于妊娠第33天固定胎盘切片的绒毛滋养层上。检测的其他固定正常狒狒组织与这些抗体无反应。发现单克隆抗体BM2可染色绒毛细胞滋养层；BM3和BM4与合体滋养层反应，但染色特征不同。免疫印迹研究表明，BM2、BM3和BM4抗体分别识别对应于30.9、31.3和42.3 kDa的抗原。
