Xu Rong, Fang Xu-Hao, Zhong Ping
Neurosurgical Department of Huashan Hospital, Fudan University, Shanghai 200040, China.
Neurosurgical Department of Huadong Hospital, Fudan University, Shanghai 200040, China.
Korean J Physiol Pharmacol. 2016 Mar;20(2):139-45. doi: 10.4196/kjpp.2016.20.2.139. Epub 2016 Feb 23.
Previously characterized as a backward motor, myosin VI (MYO6), which belongs to myosin family, moves toward the minus end of the actin track, a direction opposite to all other known myosin members. Recent researches have illuminated the role of MYO6 in human cancers, particularly in prostate cancer. However, the role of MYO6 in glioma has not yet been determined. In this study, to explore the role of MYO6 in human glioma, lentivirus-delivered short hairpin RNA (shRNA) targeting MYO6 was designed to stably down-regulate its endogenous expression in glioblastoma cells U251. Knockdown of MYO6 signifi cantly inhibited viability and proliferation of U251 cells in vitro. Moreover, the cell cycle of U251 cells was arrested at G0/G1 phase with the absence of MYO6, which could contribute to the suppression of cell proliferation. In conclusion, we firstly identified the crucial involvement of MYO6 in human glioma. The inhibition of MYO6 by shRNA might be a potential therapeutic method in human glioma.
肌球蛋白VI（MYO6）以前被描述为一种反向运动蛋白，它属于肌球蛋白家族，朝着肌动蛋白轨道的负端移动，这一方向与所有其他已知的肌球蛋白成员相反。最近的研究阐明了MYO6在人类癌症中的作用，尤其是在前列腺癌中。然而，MYO6在胶质瘤中的作用尚未确定。在本研究中，为了探究MYO6在人类胶质瘤中的作用，设计了靶向MYO6的慢病毒介导的短发夹RNA（shRNA），以稳定下调其在胶质母细胞瘤细胞U251中的内源性表达。敲低MYO6显著抑制了U251细胞在体外的活力和增殖。此外，在没有MYO6的情况下，U251细胞的细胞周期停滞在G0/G1期，这可能有助于抑制细胞增殖。总之，我们首次确定了MYO6在人类胶质瘤中的关键作用。通过shRNA抑制MYO6可能是人类胶质瘤的一种潜在治疗方法。
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