SETD6相互作用蛋白的蛋白质组学分析。
Proteomic analysis of SETD6 interacting proteins.
作者信息
Cohn Ofir, Chen Ayelet, Feldman Michal, Levy Dan
机构信息
The Shraga Segal Department of Microbiology, Immunology and Genetics, Israel; The National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, P.O.B. 653, Be׳er-Sheva, 84105 Israel.
出版信息
Data Brief. 2016 Jan 29;6:799-802. doi: 10.1016/j.dib.2016.01.042. eCollection 2016 Mar.
SETD6 (SET-domain-containing protein 6) is a mono-methyltransferase that has been shown to methylate RelA and H2AZ. Using a proteomic approach we recently identified several new SETD6 substrates. To identify novel SETD6 interacting proteins, SETD6 was immunoprecipitated (IP) from Human erythromyeloblastoid leukemia K562 cells. SETD6 binding proteins were subjected to mass-spectrometry analysis resulting in 115 new SETD6 binding candidates. STRING database was used to map the SETD6 interactome network. Network enrichment analysis of biological processes with Gene Ontology (GO) database, identified three major groups; metabolic processes, muscle contraction and protein folding.
SETD6（含SET结构域蛋白6）是一种单甲基转移酶，已被证明可使RelA和H2AZ发生甲基化。我们最近采用蛋白质组学方法鉴定了几种新的SETD6底物。为了鉴定与SETD6相互作用的新蛋白，从人红白血病K562细胞中免疫沉淀（IP）出SETD6。对SETD6结合蛋白进行质谱分析，得到115个新的SETD6结合候选蛋白。使用STRING数据库绘制SETD6相互作用组网络。利用基因本体论（GO）数据库对生物过程进行网络富集分析，确定了三个主要类别：代谢过程、肌肉收缩和蛋白质折叠。
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