Zheng Yao, Chen Jiazhang, Liu Yan, Gao Jiancao, Yang Yanping, Zhang Yingying, Bing Xuwen, Gao Zexia, Liang Hongwei, Wang Zaizhao
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China; Freshwater Fisheries Research Center, Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Chinese Academy of Fishery Sciences, China; Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Ministry of Agriculture, China; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, HZAU, Wuhan 430070, China.
Freshwater Fisheries Research Center, Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Chinese Academy of Fishery Sciences, China; Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Ministry of Agriculture, China; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
Ecotoxicol Environ Saf. 2016 Jun;128:143-52. doi: 10.1016/j.ecoenv.2015.11.034. Epub 2016 Mar 1.
The effects of synthetic androgen 17α-methyltestosterone (MT) on endocrine impairment were examined in crucian carp. Immature 7-month old mono-female Pengze crucian carp (Pcc) F2 offspring were exposed to 50 and 100 μg/L of MT (week 2, 4, and 8). Gonadosomatic index, hepatosomatic index and intestine weight altered considerably and oocyte development was repressed. In the treatment groups, ovarian 11-ketotestosterone decreased, whereas 17β-estradiol and testosterone increased, and ovarian aromatase activities increased at week 4. However, in the brain tissue, those values significantly decreased. Quantitative RT-PCR analysis demonstrated changes in steroid receptor genes and upregulation of steroidogenic genes (Pcc-3bhsd, Pcc-11bhsd2 Pcc-cyp11a1), while the other three steroidogenic genes (Pcc-cyp17a1, Pcc-cyp19a1a and Pcc-star) decreased from week 4 to week 8. Ovarian, hepatic Pcc-vtg B and vitellogenin concentration increased in both 50 and 100 μg/L of MT exposure groups. This study adds further information regarding the effects of androgens on the development of previtellogenic oocytes, which suggests that MT could directly target estrogen signaling pathway, or indirectly affect steroidogenesis and vitellogenesis.
研究了合成雄激素17α-甲基睾酮（MT）对鲫鱼内分泌损伤的影响。将7个月大的未成熟单性彭泽鲫（Pcc）F2代幼鱼暴露于50和100μg/L的MT中（第2、4和8周）。性腺指数、肝体指数和肠重发生了显著变化，卵母细胞发育受到抑制。在处理组中，卵巢11-酮睾酮减少，而17β-雌二醇和睾酮增加，且在第4周时卵巢芳香化酶活性增加。然而，在脑组织中，这些值显著降低。定量RT-PCR分析表明类固醇受体基因发生变化，类固醇生成基因（Pcc-3bhsd、Pcc-11bhsd2、Pcc-cyp11a1）上调，而其他三个类固醇生成基因（Pcc-cyp17a1、Pcc-cyp19a1a和Pcc-star）从第4周开始至第8周减少。在50和100μg/L的MT暴露组中，卵巢、肝脏中的Pcc-vtg B和卵黄蛋白原浓度均增加。本研究进一步补充了雄激素对卵黄生成前卵母细胞发育影响的信息，表明MT可能直接靶向雌激素信号通路，或间接影响类固醇生成和卵黄生成。
