Cytotoxicity assays are used for drug screening and cytotoxicity tests of chemicals. Nowadays, various reagents are used for cell viability detection. They are based on various cell functions such as enzyme activity, cell membrane permeability, cell adherence, ATP production, co-enzyme production and nucleotide uptake activity. Many have established methods such as colony formation method, crystal violet method, tritium-labelled thymidine uptake method, MTT and WST methods, which are used for counting the number of live cells. Moreover, trypan blue is a widely used assay for staining dead cells. In this method, cell viability must be determined by counting the unstained cells with a microscope or other instruments. This chapter is a collection of all these methods to be followed by researchers in a sequential manner.
细胞毒性测定用于药物筛选和化学物质的细胞毒性测试。如今，各种试剂被用于细胞活力检测。它们基于各种细胞功能，如酶活性、细胞膜通透性、细胞黏附、ATP生成、辅酶生成和核苷酸摄取活性。许多已建立的方法，如集落形成法、结晶紫法、氚标记胸腺嘧啶核苷摄取法、MTT和WST法，用于计数活细胞数量。此外，台盼蓝是一种广泛用于染色死细胞的测定方法。在该方法中，必须通过显微镜或其他仪器计数未染色的细胞来确定细胞活力。本章收集了所有这些方法，供研究人员按顺序遵循。
