Yin Fenggui, Jing Li, Zhang Shuang, Yu Meng, Zhang Wanlin, Fan Guobing, Dong Xiukai, Liu Wenjun
Sheng Wu Gong Cheng Xue Bao. 2015 Nov;31(11):1579-88.
The rapid mutation and widely spread of duck hepatitis A virus (DHAV) lead to the vast economic loss of the duck industry. To prepare and evaluate bivalent inactivated vaccine laboratory products of DHAV, 6 strains were screened from 201 DHAV-1 strains and 38 DHAV-3 strains by using serotype epidemiological analysis in most of the duck factory. Vaccine candidate strains were selected by ELD50 and LD50 tests in the 6 strains. Continuously passaged, the 5th passaged duck embryos bodies grinding fluid was selected as vaccine virus seeds. The virus seeds were treated with formaldehyde and water in oil in water (W/O/W) emulsions, making into three batches of two bivalent inactivated vaccine laboratory products. The safety test, antibody neutralization test, challenged protection and cross immune protection experiment suggested that the vaccines possessed good safety, and neutralizing antibodies were detected at 7th day and the challenged protection rate reached 90% to 100% at the 14th and 21st day. Moreover, immune duration of ducklings lasted more than five weeks. However, cross-immunity protection experiments with DHAV-SH and DHAV-FS only had 20%-30%. The two bivalent inactivated vaccine laboratory products of duck viral hepatitis were effective and reliable, providing a new method as well as a new product for DHAV prevention and control.
鸭甲型肝炎病毒（DHAV）的快速变异和广泛传播给养鸭业带来了巨大的经济损失。为制备和评价DHAV二价灭活疫苗实验室产品，通过对多数鸭场进行血清型流行病学分析，从201株DHAV - 1毒株和38株DHAV - 3毒株中筛选出6株。通过对这6株毒株进行ELD50和LD50试验筛选候选疫苗毒株。连续传代后，选取第5代鸭胚研磨液作为疫苗病毒种子。将病毒种子用甲醛处理并制成水包油包水（W/O/W）乳剂，制成三批两种DHAV二价灭活疫苗实验室产品。安全性试验、抗体中和试验、攻毒保护及交叉免疫保护试验表明，该疫苗安全性良好，7日龄可检测到中和抗体，14日龄和21日龄攻毒保护率达90%至100%。此外，雏鸭免疫期持续超过5周。然而，与DHAV - SH和DHAV - FS的交叉免疫保护试验仅有20% - 30%。两种鸭病毒性肝炎二价灭活疫苗实验室产品有效可靠，为DHAV的防控提供了新方法和新产品。
