Gismondi Alessandra, Pippo Francesca Di, Bruno Laura, Antonaroli Simonetta, Congestri Roberta
a LBA-Laboratory of Biology of Algae , Department of Biology, University of Rome "Tor Vergata" , Rome , Italy.
b CNR-IAMC, National Research Council, Institute for Coastal Marine Environment, Località Sa Mardini , Torregrande , Oristano , Italy.
Int J Phytoremediation. 2016 Sep;18(9):869-76. doi: 10.1080/15226514.2016.1156640.
In the present study a closed incubator, designed for biofilm growth on artificial substrata, was used to grow three isolates of biofilm-forming heterocytous cyanobacteria using an artificial wastewater secondary effluent as the culture medium. We evaluated biofilm efficiency in removing phosphorus, by simulating biofilm-based tertiary wastewater treatment and coupled this process with biodiesel production from the developed biomass. The three strains were able to grow in the synthetic medium and remove phosphorus in percentages, between 6 and 43%, which varied between strains and also among each strain according to the biofilm growth phase. Calothrix sp. biofilm turned out to be a good candidate for tertiary treatment, showing phosphorus reducing capacity (during the exponential biofilm growth) at the regulatory level for the treated effluent water being discharged into natural water systems. Besides phosphorus removal, the three cyanobacterial biofilms produced high quality lipids, whose profile showed promising chemical stability and combustion behavior. Further integration of the proposed processes could include the integration of oil extracted from these cyanobacterial biofilms with microalgal oil known for high monounsaturated fatty acids content, in order to enhance biodiesel cold flow characteristics.
在本研究中，使用一个为在人工基质上形成生物膜而设计的封闭式培养箱，以人工废水二级出水作为培养基，培养三株形成生物膜的异形蓝细菌分离株。通过模拟基于生物膜的三级废水处理，我们评估了生物膜去除磷的效率，并将此过程与利用所培养生物质生产生物柴油相结合。这三株菌株能够在合成培养基中生长，并去除6%至43%的磷，去除率因菌株不同以及同一菌株在生物膜生长阶段的不同而有所变化。发现在处理后排放到天然水系统的出水监管水平上，眉藻属生物膜在指数生长阶段具有除磷能力，是三级处理的良好候选者。除了去除磷之外，这三种蓝细菌生物膜还产生了高质量的脂质，其特性显示出良好的化学稳定性和燃烧性能。所提出工艺的进一步整合可包括将从这些蓝细菌生物膜中提取的油与以高单不饱和脂肪酸含量著称的微藻油相整合，以提高生物柴油的低温流动特性。
