Giachetti A, Said S I, Reynolds R C, Koniges F C
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3424-8. doi: 10.1073/pnas.74.8.3424.
The vasoactive intestinal polypeptide was present in synaptosomal (nerve ending) preparations from cerebral cortex, hypothalamus, and striatum of rat brain in higher concentrations than in these tissues as a whole. The total content and relative specific activity of the peptide increased with progressive purification of the synaptosomal fractions and generally followed the distribution of known synaptosomal constituents--dopamine, norepinephrine, and lactate dehydrogenase (L-lactate:NAD+ oxidoreductase, EC 1.1.1.27). The peptide was also released from synaptosomal pellets with increased K+ concentration, and this release was Ca2+-dependent. The findings suggest a role for vasoactive intestinal polypeptide as a transmitter or modulator of synaptic function.
血管活性肠肽在大鼠脑的大脑皮质、下丘脑和纹状体的突触体（神经末梢）制剂中的浓度高于这些组织整体的浓度。随着突触体组分的逐步纯化，该肽的总含量和相对比活性增加，并且总体上遵循已知突触体成分——多巴胺、去甲肾上腺素和乳酸脱氢酶（L-乳酸：NAD⁺氧化还原酶，EC 1.1.1.27）的分布。该肽也随着钾离子浓度升高而从突触体沉淀中释放出来，并且这种释放是钙离子依赖性的。这些发现表明血管活性肠肽作为突触功能的递质或调节剂发挥作用。
