Ding Cuicui, Zheng Zhigong, Liu Xinzhong, Li Hengda, Zhang Min
a College of Ecological Environment and Urban Construction , Fujian University of Technology , Fuzhou , China.
b College of Materials Engineering , Fujian Agriculture and Forestry University , Fuzhou , China.
Connect Tissue Res. 2016 Jul;57(4):270-6. doi: 10.3109/03008207.2016.1149170. Epub 2016 Mar 3.
The effect of γ-poly(glutamic acid) (γ-PGA) on the self-assembly of collagen was studied. Under physiological conditions, the kinetic curves for fibril formation showed that the turbidity of collagen/γ-PGA blends at 313 nm was increased with the addition of γ-PGA. Furthermore, it was shown using both field-emission scanning electron microscopy (FESEM) and atomic force microscopy (AFM) that fibrils with a larger diameter were obtained following the addition of γ-PGA, probably due to the electrostatic and hydrogen bond interactions between collagen and γ-PGA, which promoted the lateral association of collagen molecules. In addition, both the thermal stability and viscoelastic properties of the hybrid hydrogels, which were evaluated by differential scanning calorimetry and rheological measurements, respectively, were improved by the addition of γ-PGA.
研究了γ-聚谷氨酸（γ-PGA）对胶原蛋白自组装的影响。在生理条件下，原纤维形成的动力学曲线表明，添加γ-PGA后，胶原蛋白/γ-PGA混合物在313nm处的浊度增加。此外，用场发射扫描电子显微镜（FESEM）和原子力显微镜（AFM）均表明，添加γ-PGA后可得到直径更大的原纤维，这可能是由于胶原蛋白与γ-PGA之间的静电和氢键相互作用促进了胶原蛋白分子的侧向缔合。另外，分别通过差示扫描量热法和流变学测量评估的杂化水凝胶的热稳定性和粘弹性，均因添加γ-PGA而得到改善。
