在添加去脂血清的培养基中神经母细胞瘤细胞的形态分化
Morphological differentiation of neuroblastoma cells in medium supplemented with delipidated serum.
作者信息
Monard D, Rentsch M, Schuerch-Rathgeb Y, Lindsay R M
出版信息
Proc Natl Acad Sci U S A. 1977 Sep;74(9):3893-7. doi: 10.1073/pnas.74.9.3893.
Abstract
Neuroblastoma cells have been found to extend neurites when grown in a medium supplemented with delipidated fetal calf serum. Fetal calf sera from different commercial sources give rise to marked differences in the degree of this spontaneous morphological differentiation. The phenomenon can be prevented by the addition of certain fatty acids; oleic acid is especially effective. The serum-free conditioned medium from glial cells can quantitatively antagonize the effect of oleic acid, suggesting that glial factor activity could be due to components [lipids and/or macromolecular factors(s)] that are able to modify the properties of the neuroblastoma cell membrane.
摘要
已发现神经母细胞瘤细胞在添加了脱脂胎牛血清的培养基中生长时会延伸神经突。来自不同商业来源的胎牛血清在这种自发形态分化的程度上产生显著差异。添加某些脂肪酸可以阻止这种现象；油酸尤其有效。来自神经胶质细胞的无血清条件培养基可以定量拮抗油酸的作用，这表明神经胶质因子活性可能归因于能够改变神经母细胞瘤细胞膜特性的成分[脂质和/或大分子因子]。
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2
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J Clin Invest. 1955 Sep;34(9):1345-53. doi: 10.1172/JCI103182.
3
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